玉米胚乳和盾片的己糖激酶。
Hexokinase from maize endosperm and scutellum.
机构信息
Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
出版信息
Plant Physiol. 1973 May;51(5):960-6. doi: 10.1104/pp.51.5.960.
Abstract
Hexokinase (EC 2.7.1.1) was isolated from endosperm and scutellum of developing and germinating maize (Zea mays) seeds. With fructose as the variable substate, Michaelis constant values for the scutellum enzyme were about onethird those of the endosperm enzyme (0.05 versus 0.15 mm), and no developmental differences were observed. With glucose as the variable substrate, Michaelis constant values were all in the range 0.1 to 0.2 mm. The enzyme preparation from germinating scutellum was studied further; when glucose was varied over a wide range, a Michaelis constant of 3.4 mm was observed in addition to the much lower Michaelis constant noted above. This low affinity binding of glucose may have regulatory significance and may indicate the presence of a glucokinase in addition to hexokinase.
摘要
己糖激酶（EC 2.7.1.1）从发育和萌发的玉米（Zea mays）种子的胚乳和盾片中分离出来。以果糖作为可变量的亚基，盾片酶的米氏常数大约是胚乳酶的三分之一（0.05 对 0.15mm），并且没有观察到发育差异。以葡萄糖作为可变量的底物，米氏常数都在 0.1 到 0.2mm 范围内。进一步研究了萌发的盾片中的酶制剂；当葡萄糖在很宽的范围内变化时，除了上述的低米氏常数外，还观察到 3.4mm 的米氏常数。这种葡萄糖的低亲和力结合可能具有调节意义，并可能表明除了己糖激酶之外还存在葡萄糖激酶。
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