Department of Chemistry, Plant Physiology-Biochemistry Group, State University of New York, College of Environmental Science and Forestry, Syracuse, New York 13210.
Plant Physiol. 1973 Jun;51(6):1147-9. doi: 10.1104/pp.51.6.1147.
Soluble proteins from excised Phaseolus vulgaris axes incubated for 1 hour in (3)H or (14)C- amino acid mixtures at different times during the period leading up to initiation of cell elongation were compared by acrylamide gel electrophoresis. Differences in electrophoretic patterns were found when proteins from axes incubated during the 1st hour of imbibition were compared with proteins from axes incubated during the hour when cell elongation was initiated. These differences greatly diminished by the 2nd hour of imbibition which suggests that they were due primarily to incomplete axis imbibition. A 5-hour actinomycin D treatment which reduced amino acid incorporation by 40% in the 5th hour had no apparent effect on the electrophoretic pattern during that hour.
从不同时间用 (3)H 或 (14)C-氨基酸混合物处理的外植豌豆轴中切取的可溶性蛋白，在诱导细胞伸长的时期内孵育 1 小时后，通过丙烯酰胺凝胶电泳进行比较。当比较在吸胀的第 1 小时孵育的轴中的蛋白质与在细胞伸长开始的小时孵育的轴中的蛋白质时，发现电泳图谱存在差异。这些差异在吸胀的第 2 小时大大减少，这表明它们主要是由于不完全的轴吸胀。5 小时放线菌素 D 处理使第 5 小时的氨基酸掺入减少 40%，但对该小时的电泳图谱没有明显影响。
