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大麦种子中5'-磷酰氟腺苷的形成及腺苷酸环化酶的测定

Formation of adenosine 5'-phosphorofluoridate and the assay of adenyl cyclase in barley seeds.

作者信息

Alvarez R

机构信息

Institute of Biological Sciences, Syntex Research, Palo Alto, California 94304.

出版信息

Plant Physiol. 1974 Feb;53(2):144-8. doi: 10.1104/pp.53.2.144.

DOI:10.1104/pp.53.2.144
PMID:16658665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC541353/
Abstract

Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5'-phosphorofluoridate from magnesium-adenosine 5'-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3': 5'-monophosphate was detected in barley seed homogenates or extracts.

摘要

大麦种子(Hordeum vulgare L.)匀浆含有一种明显的酶活性,可催化由镁 - 腺苷5'-三磷酸和氟化钠合成5'-磷酰氟代腺苷。该化合物的形成可能会干扰一些使用氟化物作为孵育培养基成分的腺苷酸环化酶测定。在大麦种子匀浆或提取物中未检测到腺苷酸环化酶活性或内源性腺苷3':5'-单磷酸。

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本文引用的文献

1
Cyclic nucleotide phosphodiesterase activity in barley seeds.大麦种子中环核苷酸磷酸二酯酶活性。
Plant Physiol. 1973 Sep;52(3):278-82. doi: 10.1104/pp.52.3.278.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Adenyl cylase. I. Distribution, preparation, and properties.腺苷酸环化酶。I. 分布、制备及性质。
J Biol Chem. 1962 Apr;237:1220-7.
4
Nucleotide synthesis. II. Nucleotide p-nitrophenyl and 2,4-dinitrophenyl esters.核苷酸合成。II. 核苷酸对硝基苯酯和2,4-二硝基苯酯。
J Org Chem. 1966 Oct;31(10):3241-6. doi: 10.1021/jo01348a034.
5
Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom.环核苷酸依赖性蛋白激酶。IV. 3',5'-环磷酸腺苷依赖性蛋白激酶在动物界各组织和门类中的广泛存在。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349-55. doi: 10.1073/pnas.64.4.1349.
6
Cyclic nucleotide-independent protein kinase from pea shoots.来自豌豆芽的不依赖环核苷酸的蛋白激酶。
Biochem Biophys Res Commun. 1973 Sep 18;54(2):655-61. doi: 10.1016/0006-291x(73)91473-3.
7
Cyclic nucleotide phosphodiesterase in pea seedlings.豌豆幼苗中的环核苷酸磷酸二酯酶。
Biochim Biophys Acta. 1972 Aug 28;276(2):454-74. doi: 10.1016/0005-2744(72)91007-8.
8
The inhibition of plant and animal adenosine 3':5'-cyclic monophosphate phosphodiesterases by a cell-division-promoting substance from tissues of higher plant species.高等植物物种组织中一种促进细胞分裂的物质对植物和动物腺苷3':5'-环磷酸磷酸二酯酶的抑制作用
Proc Natl Acad Sci U S A. 1972 Feb;69(2):403-6. doi: 10.1073/pnas.69.2.403.
9
Rapid gibberellin responses and the action of adenosine 3',5'-monophosphate in aleurone layers.赤霉素的快速反应以及腺苷 3',5'-单磷酸在糊粉层中的作用。
Biochim Biophys Acta. 1971 Dec 21;252(3):553-60. doi: 10.1016/0304-4165(71)90158-9.
10
Auxin-induced synthesis of cyclic 3', 5'-adenosine monophosphate in Avena coleoptiles.生长素诱导燕麦胚芽鞘中3',5'-环磷酸腺苷的合成。
Life Sci II. 1971 Aug;10(15):879-85. doi: 10.1016/0024-3205(71)90200-1.