大麦种子中5'-磷酰氟腺苷的形成及腺苷酸环化酶的测定
Formation of adenosine 5'-phosphorofluoridate and the assay of adenyl cyclase in barley seeds.
作者信息
Alvarez R
机构信息
Institute of Biological Sciences, Syntex Research, Palo Alto, California 94304.
出版信息
Plant Physiol. 1974 Feb;53(2):144-8. doi: 10.1104/pp.53.2.144.
Abstract
Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5'-phosphorofluoridate from magnesium-adenosine 5'-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3': 5'-monophosphate was detected in barley seed homogenates or extracts.
摘要
大麦种子（Hordeum vulgare L.）匀浆含有一种明显的酶活性，可催化由镁 - 腺苷5'-三磷酸和氟化钠合成5'-磷酰氟代腺苷。该化合物的形成可能会干扰一些使用氟化物作为孵育培养基成分的腺苷酸环化酶测定。在大麦种子匀浆或提取物中未检测到腺苷酸环化酶活性或内源性腺苷3':5'-单磷酸。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Nucleotide synthesis. II. Nucleotide p-nitrophenyl and 2,4-dinitrophenyl esters.核苷酸合成。II. 核苷酸对硝基苯酯和2,4-二硝基苯酯。
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