Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Plant Physiol. 1973 Dec;52(6):633-7. doi: 10.1104/pp.52.6.633.
Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.The energy-dependent transport of glycine was mediated by a carrier that had a broad specificity for neutral and positively charged l-amino acids. It readily translocated 15 such l-amino acids into the cells, but had a very low affinity for l-aspartate, l-glutamate, d-amino acids, and alpha-aminoisobutyrate. The Ki for competitive inhibition of glycine uptake by another amino acid was equal to the Km for the uptake of that competing species.
豌豆叶片碎片对氨基酸的摄取依赖于代谢。甘氨酸的最佳摄取温度为 30°C,可以通过呼吸作用和光合作用来支持。基于电子流动辅助因子、抑制剂和解偶联剂的研究,甘氨酸摄取的能量来源显然是 ATP。甘氨酸的能量依赖性运输是由一种载体介导的,该载体对中性和带正电荷的 l-氨基酸具有广泛的特异性。它很容易将 15 种这样的 l-氨基酸转运到细胞中,但对 l-天冬氨酸、l-谷氨酸、d-氨基酸和 α-氨基异丁酸的亲和力很低。另一种氨基酸对甘氨酸摄取的竞争性抑制的 Ki 等于该竞争种摄取的 Km。