Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours & Company, Wilmington, Delaware 19898.
Plant Physiol. 1986 May;81(1):8-11. doi: 10.1104/pp.81.1.8.
We isolated large quantities of mesophyll protoplasts from source and sink leaves of soybean plants and examined them for amino acid uptake. Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes. Uptake kinetics revealed the presence of both saturable and linear components. Increasing external pH decreases the uptake. The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake. Our data are consistent with a proton-cotransport mechanism for the uptake of l-glutamine and alpha-amino isobutyric acid into soybean mesophyll cells.
我们从大豆植株的源叶和汇叶中分离出大量的叶肉原生质体，并检查它们对氨基酸的吸收情况。分离的原生质体中氨基酸的积累至少在 40 分钟内呈线性。摄取动力学显示存在饱和和线性成分。增加外部 pH 值会降低摄取。在 15 微摩尔的羰基氰化物 p-三氟甲氧基苯腙抑制和在 10 微摩尔的 Fusicoccin 刺激氨基酸摄取。我们的数据与质子共转运机制一致，用于将 l-谷氨酰胺和α-氨基异丁酸摄取到大豆叶肉细胞中。
