Biological Institute, Faculty of Science, Nagoya University, Chikusa, Nagoya 464, Japan.
Plant Physiol. 1974 Mar;53(3):343-7. doi: 10.1104/pp.53.3.343.
When mitochondria isolated from etiolated pea (Pisum sativum cv. Alaska) epicotyls were exposed briefly to red light, their ability to reduce exogenous NADP was enhanced. The red light effect was reversed by far red light. Photoreversible absorbance changes between 730 nm and 800 nm were spectrophotometrically detected in the purified mitochondria and its membrane fraction. The dehydrogenase activity in the mitochondria was heat-labile and was dependent on the presence of magnesium ion and appropriate substrates such as glucose 6-phosphate, isocitrate, pyruvate, 6-phosphogluconate, and succinate. The photoreversible effect was seen only for a few minutes after the irradiation, and was cancelled by hypotonic treatment or addition of Triton X-100. A similar but lesser effect was observed in the pea microsome fraction, whereas no photoreversible response was seen with a supernatant fraction resulting from centrifugation at 10(5)g for 30 minutes.
当从黄化豌豆（Pisum sativum cv. Alaska）上胚轴分离的线粒体短暂暴露于红光下时，它们还原外源 NADP 的能力增强。红光效应可被远红光逆转。在纯化的线粒体及其膜部分中分光光度法检测到 730nm 和 800nm 之间的光可逆吸收变化。线粒体中的脱氢酶活性是热不稳定的，并且依赖于镁离子和适当的底物如葡萄糖 6-磷酸、异柠檬酸、丙酮酸、6-磷酸葡萄糖酸和琥珀酸的存在。照射后仅几分钟即可观察到光可逆效应，并且通过低渗处理或添加 Triton X-100 可将其消除。在豌豆微粒体部分中观察到类似但较小的效应，而在用 10(5)g 离心 30 分钟产生的上清部分中则未观察到光可逆反应。
