Department of Biology, Vanderbilt University, 37235, Nashville, Tennessee, USA.
Planta. 1974 Jan;121(2):119-31. doi: 10.1007/BF00388751.
An immunocytochemical technique was used to localize the red-absorbing form of phytochrome at the light- or electron-microscope level in etiolated barley (Hordeum vulgare L.) coleoptile tip, rice (Oryza sativa L.) coleoptilar node, maize (Zea mays L.) coleoptile tip, rye (Secale cereale L.) coleoptile tip and coleoptilar node, and oat (Avena sativa L.) root cap. Staining for phytochrome in the cells was found to be generally distributed throughout the cytoplasm. In addition, barley also showed staining around the periphery of vesicles, and rice showed staining in numerous discrete regions in the cytoplasm. Electron-microscopic localization studies of the nodal region of rye and the root cap of oat indicate staining associated with the nuclear membrane and with the interior of mitochondria and amyloplasts as well as general staining like that observed with the light microscope. Cells of the coleoptile tip of maize were unusual in having heavy staining associated with amyloplasts only.
采用免疫细胞化学技术，在暗中生长的大麦（Hordeum vulgare L.）胚芽鞘顶端、水稻（Oryza sativa L.）胚芽鞘节、玉米（Zea mays L.）胚芽鞘顶端、黑麦（Secale cereale L.）胚芽鞘顶端和胚芽鞘节以及燕麦（Avena sativa L.）根冠中，对红光吸收型光敏素进行了光镜或电镜水平的定位。在细胞中发现，光敏素的染色通常分布在整个细胞质中。此外，大麦还显示出围绕小泡周围的染色，而水稻则显示出细胞质中许多离散区域的染色。黑麦节和燕麦根冠的电子显微镜定位研究表明，染色与核膜以及线粒体和淀粉体的内部有关，并且与用显微镜观察到的一般染色相似。玉米胚芽鞘顶端的细胞很不寻常，只有与淀粉体相关的强烈染色。
