Department of Botany, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan.
Planta. 1975 Jan;127(2):177-86. doi: 10.1007/BF00388379.
If the 1000×g supernatant from a homogenate of etiolated pea (Pisum sativum L. cv. Alaska) epicotyls is briefly irradiated with red light at ca. 0° C, the increase of phytochrome content in the 1000-7000×g particulate fraction is strongly dependent upon the Ca(2+)concentration, being highest at 10 mM. This Ca(2+)-dependent binding of phytochrome to the particulate fraction in the supernatant is partially red/far-red reversible. Mg(2+) is significantly less effective than Ca(2+) while Na(+) and K(+) have no effect.The 80 000×g supernatant (S) and the 1000-7000×g particulate fraction (P) were prepared and separated from a pea homogenate. When either S or P were briefly exposed to red light and then mixed together at 0° C in darkness and the presence of 10 mM Ca(2+), the amount of phytochrome bound to the 7000×g pellet increased immediately. This increase was 30% after mixing irradiated S with dark P, 18% after mixing irradiated P with dark S and 55% after both S and P had been irradiated and mixed. In all cases, the red light effect is totally far-red reversible.
如果将黑暗中培养的豌豆（Pisum sativum L. cv. Alaska）下胚轴匀浆的 1000xg 上清液在约 0°C 下短暂照射红光，则 1000-7000xg 颗粒部分中光敏素含量的增加强烈依赖于 Ca(2+)浓度，在 10 mM 时最高。这种上清液中光敏素与颗粒部分的 Ca(2+)依赖性结合在部分红/远红光下是可逆的。Mg(2+)的效果明显低于 Ca(2+)，而 Na(+)和 K(+)则没有影响。从豌豆匀浆中制备并分离出 80 000xg 上清液（S）和 1000-7000xg 颗粒部分（P）。当 S 或 P 短暂暴露于红光中，然后在黑暗中和存在 10 mM Ca(2+)的情况下在 0°C 下混合时，结合到 7000xg 沉淀上的光敏素的量立即增加。用辐照 S 与黑暗 P 混合后增加 30%，用辐照 P 与黑暗 S 混合后增加 18%，用辐照 S 和 P 混合后增加 55%。在所有情况下，红光效应完全是远红光可逆的。
