Biological Institute, Faculty of Science, Nagoya University, Chikusa, 464, Nagoya, Japan.
Planta. 1975 Jan;123(3):207-15. doi: 10.1007/BF00390699.
A brief irradiation with red light of pea (Pisum sativum L.) shoot segments kept at 0° resulted in very rapid binding of both Pr and Pfr to mitochondrial and microsomal fractions. The effect was not far-red reversible. The amount of phytochrome bound to the mitochondrial fraction was proportional to the percentage of Pfr of the fraction, and the ratio of Pr and Pfr in the bound form was the same as that in 12,000 x g supernatant. After a brief exposure of the segments to red light at 0° and a subsequent dark incubation at 30° in Tris-HCL buffer containing dithiothreitol or EDTA, which bot inhibit Pfr decay, the contents of phytochrome in the mitochondrial and microsomal fractions were significantly enhanced with time. The red-light effect was reversed by far-red light. The increase of the phytochrome content in the particulate fractions continued for at least 2 h, reaching a ca. 3 times higher level in terms of Δ (ΔA) per mg protein.
将豌豆（Pisum sativum L.）茎段在 0°C 下短暂红光照射，导致 Pr 和 Pfr 迅速结合到线粒体和微粒体部分。该效应不能被远红光逆转。与线粒体部分结合的光敏素的量与该部分 Pfr 的百分比成正比，结合形式中的 Pr 和 Pfr 的比例与 12000 x g 上清液中的相同。在 0°C 下短暂暴露于红光后，在含有二硫苏糖醇或 EDTA 的 Tris-HCL 缓冲液中于 30°C 下进行黑暗孵育，这两种物质均抑制 Pfr 衰减，随后线粒体和微粒体部分中光敏素的含量随时间显著增加。红光效应可被远红光逆转。颗粒部分中光敏素含量的增加至少持续 2 小时，以每毫克蛋白质的 Δ（ΔA）计，达到约 3 倍的更高水平。
