Post-Harvest Plant Physiology Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705.
Plant Physiol. 1974 May;53(5):768-71. doi: 10.1104/pp.53.5.768.
Seeds sterilized with sodium hypochlorite (NaOCl) retained sufficient amounts to interfere with studies of amino acid metabolism of the sterilized seeds during germination. Repeated washing in water did not remove NaOCl completely. However, soaking the seeds for 10 min in 0.01 n HCl removed NaOCl completely, without reducing germinability.Residual NaOCl reacted with the amino acids and reduced their concentrations in the incubation media. This reaction resulted in high production of CO(2) and low uptake of amino acids by the seeds. Decarboxylation of the amino acids occurred in the incubation medium outside the seed, was independent of the presence of seeds in the reaction, and therefore was not related to amino acid metabolism by the seeds. Effects of NaOCl on uptake, incorporation, and CO(2) production from indoleacetic acid were similar to those of the amino acids studied.
用次氯酸钠（NaOCl）消毒的种子保留了足够的次氯酸钠，从而干扰了消毒种子在萌发过程中氨基酸代谢的研究。用清水反复冲洗不能完全去除次氯酸钠。然而，将种子在 0.01n HCl 中浸泡 10 分钟可以完全去除次氯酸钠，而不降低发芽率。残留的次氯酸钠与氨基酸反应，降低了它们在孵育介质中的浓度。这种反应导致种子产生大量的二氧化碳和对氨基酸的低吸收。氨基酸在种子外的孵育介质中脱羧，与反应中是否存在种子无关，因此与种子的氨基酸代谢无关。次氯酸钠对吲哚乙酸的吸收、掺入和二氧化碳产生的影响与所研究的氨基酸相似。
