Chun S-C, Schneider R W, Cohn M A
Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge 70803.
Plant Dis. 1997 Jul;81(7):821-824. doi: 10.1094/PDIS.1997.81.7.821.
Bacteria were completely eliminated from rice seeds following immersion in household bleach solutions (50% bleach and 2.6% NaOCl) adjusted to pH 7.0 in 0.5 M potassium phosphate, while fungi were eliminated at pH 5.0 and below. Mercuric chloride was used to disinfest non-dormant rice seeds in order to test the direct effect of sodium hypochlorite on rice seedling growth apart from an indirect effect related to elimination of microbial contaminants. Seeds were immersed for 2 h in each solution or water in each of the following treatments: (i) sterilewater followed by sterile water, (ii) HgCl (1,000 μg/ml) followed by sterile water, (iii) HgCl followed by KHPO (0.3 M), (iv) HgCl followed by NaOCl, and (v) NaOCl followed by sterile water. Sodium hypochlorite solutions were prepared in 0.3 M potassium phosphate, which gave a final pH of 7.3. Seedling growth in treatments in which seeds were treated with HgCl followed by NaOCl, and NaOCl followed by sterile water, was significantly greater than those treatments in which seeds were treated with sterile water followed by sterile water, HgCl followed by sterile water, and HgCl followed by KHPO. Sodium hypochlorite stimulated rice seedling growth directly, as opposed to an indirect effect related to elimination of microbial contaminants or alleviation of dormancy.
将水稻种子浸泡在经0.5M磷酸钾调节至pH 7.0的家用漂白剂溶液（50%漂白剂和2.6%次氯酸钠）中后，细菌被完全清除，而真菌在pH 5.0及以下时被清除。使用氯化汞对非休眠水稻种子进行消毒，以测试次氯酸钠对水稻幼苗生长的直接影响，而不是与消除微生物污染物相关的间接影响。在以下每种处理中，将种子在每种溶液或水中浸泡2小时：（i）无菌水后接无菌水，（ii）HgCl（1000μg/ml）后接无菌水，（iii）HgCl后接KHPO（0.3M），（iv）HgCl后接次氯酸钠，以及（v）次氯酸钠后接无菌水。次氯酸钠溶液用0.3M磷酸钾配制，最终pH为7.3。种子先经HgCl处理后接次氯酸钠，以及先经次氯酸钠处理后接无菌水的处理中，幼苗生长显著大于种子先经无菌水后接无菌水、HgCl后接无菌水以及HgCl后接KHPO的处理。次氯酸钠直接刺激水稻幼苗生长，这与消除微生物污染物或缓解休眠的间接影响相反。
