Department of Biological Sciences, Madurai University, Madurai, 21, India.
Plant Physiol. 1974 Oct;54(4):569-74. doi: 10.1104/pp.54.4.569.
A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Crotalaria Laburnifolia L., and Thunbergia grandiflora Roxb.By pulse-chase time course experiments, the photosynthetically fixed primary carbon compounds of bean leaf cells were not converted into the ethanol-insoluble fraction. About 25% of total (14)C-photoassimilates were found to leak out into the incubation medium. In contrast, Euglena and Chlorella cells incorporated their primary photosynthetic products into cellular macromolecules and the amount of "leak" was very little. (14)C-Leucine supplied to the bean cells was absorbed readily and incorporated into the trichloroacetic acid insoluble fraction.
已经对许多植物进行了研究，以了解在大量叶细胞中通过轻度研磨进行分离的情况。发现每单位叶面积中叶肉细胞的回收程度因植物种类和研磨方法而异。从美人蕉、鸡屎藤和大花老鸭嘴的叶片中可获得超过 70%的回收。通过脉冲追踪时间过程实验，发现豆叶细胞中光合作用固定的初级碳化合物不会转化为乙醇不溶部分。约 25%的总 (14)C-光产物被发现渗漏到培养介质中。相比之下，衣藻和小球藻细胞将其初级光合作用产物掺入细胞大分子中，“渗漏”的量非常少。(14)C-亮氨酸很容易被豆细胞吸收并掺入三氯乙酸不溶部分。
