Centre de Physiologie Végétale, Laboratoire Associé au CNRS no 241, Université Paul Sabatier 118, Ioute de Narbonne, 31062, Toulouse Cédex, France.
Photosynth Res. 1982 Jan;3(1):59-67. doi: 10.1007/BF00030050.
Mesophyll cells were isolated from sunflower leaves by an enzymic procedure. The cell suspensions possessed high photosynthesis rates. The products of cell photosynthesis were similar to the products of leaf disc photosynthesis. The relatively high radioactivity incorporated into malate after (14)CO2 feeding suggests that PEP carboxylase might participate in CO2 fixation. Sunflower leaf extracts possessed a PEP carboxylase activity slightly higher than that of other C3 species. Inhibition of PEP carboxylase by maleate decreased cell photosynthesis by only 15% and the first products of cell photosynthesis were phosphorylated compounds. It is concluded that the high photosynthesis rates displayed by sunflower are not due to a parallel C4 pathway of photosynthesis but are rather dependent, at least in part, on the activity, or the amount, of RuBP carboxylase.
通过酶处理从向日葵叶片中分离出叶肉细胞。细胞悬浮液具有较高的光合作用速率。细胞光合作用的产物与叶片圆盘光合作用的产物相似。（14）CO2 喂养后掺入的相对较高的放射性物质表明 PEP 羧化酶可能参与 CO2 固定。向日葵叶片提取物的 PEP 羧化酶活性略高于其他 C3 物种。马来酸抑制 PEP 羧化酶仅使细胞光合作用降低 15%，细胞光合作用的最初产物是磷酸化化合物。由此得出结论，向日葵表现出的高光合作用速率不是由于平行的 C4 光合作用途径，而是至少部分依赖于 RuBP 羧化酶的活性或数量。
