The Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan.
Plant Physiol. 1974 Nov;54(5):722-4. doi: 10.1104/pp.54.5.722.
Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) which were immersed in liquid nitrogen after prefreezing to the temperatures from -30 to -50 C in the presence of dimethylsulfoxide and glucose as cryoprotective additive could proliferate vigorously when rewarmed rapidly in water at 40 C. For maintaining high viability of the cells after immersion in liquid nitrogen, it seems to be essential to use the cells at the later lag phase or the early cell division phase. This study provides a possibility for long term preservation in liquid nitrogen of plant-cultured lines.
悬浮培养的悬铃木（Acer pseudoplatanus L.）细胞在含有二甲亚砜和葡萄糖作为冷冻保护剂的情况下，预冷冻至-30 至-50°C 后浸入液氮中，当在 40°C 的水中快速复温时可以旺盛增殖。为了保持细胞在浸入液氮后的高存活率，似乎必须在细胞进入迟滞期后期或早期细胞分裂期时使用细胞。本研究为植物培养系的长期液氮保存提供了可能性。
