Department of Horticulture, Purdue University, Lafayette, Indiana 47906.
Plant Physiol. 1967 Oct;42(10):1423-6. doi: 10.1104/pp.42.10.1423.
The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride has been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue survival at a later date. An advantage of the method described here is that a small amount of tissue (50-100 mg) is required; this, therefore, allows for hardiness determinations at precise locations on the plant. The high correlation between cold injury and triphenyl tetrazolium chloride reduction may be due to cofactor and substrate limitations rather than inactivation of dehydrogenases.
采用氯化三苯基四氮唑评估木本植物冷害的方法已得到改进，以消除与视觉区分不同程度的四唑还原相关的偏差，并预测日后的组织存活情况。此处所述方法的一个优点是所需组织量较少（50-100mg）；因此，可在植物的精确位置进行抗寒性测定。冷害与氯化三苯基四氮唑还原之间的高度相关性可能是由于辅因子和底物的限制，而不是脱氢酶的失活。
