Walker G C, Leonard N J
School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1974 Nov;54(5):737-43. doi: 10.1104/pp.54.5.737.
Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N(6)-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with (3)H and in the 8-position of the purine ring with (14)C. The (3)H/(14)C ratio of N(6)-benzyladenosine recovered from labeled tobacco callus transfer RNA preparations was compared with the corresponding ratio for N(6)-benzyladenine supplied in the medium. By this means, with suitable controls, the incorporation of the synthetic cytokinin, N(6)-benzyladenine, into the transfer RNA of tobacco callus tissue was shown to involve the intact moiety. The observed level of incorporation was up to one N(6)-benzyladenosine molecule per 10(4) transfer RNA molecules.
在含有用³H标记苯环且用¹⁴C标记嘌呤环8位的N⁶ - 苄基腺嘌呤（6 - 苄基氨基嘌呤）的条件下培养依赖细胞分裂素的烟草（烟草）组织。将从标记烟草愈伤组织转移RNA制剂中回收的N⁶ - 苄基腺苷的³H/¹⁴C比值与培养基中供应的N⁶ - 苄基腺嘌呤的相应比值进行比较。通过这种方法，并设置合适的对照，结果表明合成细胞分裂素N⁶ - 苄基腺嘌呤掺入烟草愈伤组织组织的转移RNA中涉及完整部分。观察到的掺入水平高达每10⁴个转移RNA分子中有一个N⁶ - 苄基腺苷分子。
