Department of Botany, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1974 Nov;54(5):799-801. doi: 10.1104/pp.54.5.799.
Washing of corn root tissue increases the electrical potential difference, negative inside, across the membranes of epidermal cells. There is no lag period in the development of the extra potential, and the entire increase is electrogenic as evidenced by collapse with the uncoupler, (p-trifluoromethoxy)-carbonyl cyanide-phenylhydrazone.Proton extrusion by the tissue declines with washing but can be reinstituted by the addition of mersalyl, an inhibitor of the Pi-OH(-) antiporter of mitochondrial membranes.It appears that washing may enhance or augment the activity of an electrogenic ion pump, possibly a proton or cation efflux pump. In addition, there may be augmentation of an anion-OH(-) antiporter driven by the proton motive force created.
玉米根组织的洗涤会增加细胞膜的电势能差，内部为负。额外电势的产生没有滞后期，整个增加过程是电生成的，这可以通过解偶联剂（p-三氟甲氧基）-羰基氰基苯腙的崩溃来证明。组织中质子的外排随着洗涤而减少，但可以通过添加 mersalyl（一种线粒体膜 Pi-OH(-)反向转运蛋白的抑制剂）来重新建立。看来，洗涤可能会增强或增强电致离子泵的活性，可能是质子或阳离子外排泵。此外，可能会增强由质子动力势驱动的阴离子-OH(-)反向转运蛋白的活性。
