Department of Botany, North Carolina State University, 27695-7612, Raleigh, North Carolina.
J Chem Ecol. 1991 May;17(5):865-86. doi: 10.1007/BF01395596.
To determine the relative sensitivities of tomato, cucumber, and bean to exogenously applied concentrations of ferulic acid (FA) and to determine whether FA-induced stress responses increase endogenous levels of abscisic acid (ABA), wild-type andFlacca (ABA-deficient mutant) tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig), cucumber, (Cucumis sativus L. cv. Early Green Cluster), and bean (Phaseolus vulgaris L. cv. Oregon 91) were treated with FA (0.0, 0.2, 0.4, 0.8 mM) in nutrient solution every other day for a total of two or three treatments. FA inhibited leaf growth and water utilization of wild-type tomato,Flacca tomato, and cucumber, but not of bean. Acclimation to FA was observed following the first FA treatment and increased endogenous ABA levels were found in wild-type tomato,Flacca tomato, and cucumber following multiple FA treatments. Induction of ABA biosynthesis occurred in wild-type tomato within 8 hr of FA treatment and maximum ABA levels were observed 24 hr after treatment. At that time, ABA levels of tomato treated with 0.4 and 0.8 mM FA were 13.7 times and 2.6 times higher than control levels, respectively. A second FA (0.4 or 0.8 mM) treatment, 48 hr after the first, did not appear to affect ABA levels. Ninety-six hours after the first treatment, ABA levels of tomato treated with 0.4 mM FA approached control levels; ABA levels of plants treated with 0.8 mM FA were 1.9 times higher than control levels. Control ABA levels increased gradually with time. The data showed that plant sensitivity and ability of subsequent acclimation to phenolic acids, such as FA, were taxa dependent.
为了确定番茄、黄瓜和菜豆对外源阿魏酸（FA）施加浓度的相对敏感性，并确定 FA 诱导的应激反应是否会增加内源脱落酸（ABA）的水平，我们用 FA（0.0、0.2、0.4、0.8 mM）处理野生型和 Flacca（ABA 缺陷型突变体）番茄（Lycopersicon esculentum Mill. cv. Ailsa Craig）、黄瓜（Cucumis sativus L. cv. Early Green Cluster）和菜豆（Phaseolus vulgaris L. cv. Oregon 91），每隔一天在营养液中处理一次，总共进行两次或三次处理。FA 抑制了野生型番茄、Flacca 番茄和黄瓜的叶片生长和水分利用，但对菜豆没有影响。第一次 FA 处理后观察到了对 FA 的适应，并且在多次 FA 处理后，在野生型番茄、Flacca 番茄和黄瓜中发现了内源 ABA 水平的增加。ABA 生物合成在 FA 处理后 8 小时内诱导发生，并且在处理后 24 小时观察到最大的 ABA 水平。此时，用 0.4 和 0.8 mM FA 处理的番茄的 ABA 水平分别比对照水平高 13.7 倍和 2.6 倍。第二次 FA（0.4 或 0.8 mM）处理在第一次处理后 48 小时进行，似乎没有影响 ABA 水平。在第一次处理后 96 小时，用 0.4 mM FA 处理的番茄的 ABA 水平接近对照水平；用 0.8 mM FA 处理的植物的 ABA 水平比对照水平高 1.9 倍。对照 ABA 水平随时间逐渐增加。数据表明，植物对酚酸（如 FA）的敏感性和随后适应的能力取决于分类群。
