Ciniglia Claudia, Mastrobuoni Francesco, Scortichini Marco, Petriccione Milena
Department of Environmental, Biological and Pharmaceutical Science and Technology Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy.
Ecotoxicology. 2015 May;24(4):926-37. doi: 10.1007/s10646-015-1435-7. Epub 2015 Mar 4.
The allelochemical stress on Zea mays was analyzed by using walnut husk washing waters (WHWW), a by-product of Juglans regia post-harvest process, which possesses strong allelopathic potential and phytotoxic effects. Oxidative damage and cell-programmed death were induced by WHWW in roots of maize seedlings. Treatment induced ROS burst, with excess of H2O2 content. Enzymatic activities of catalase were strongly increased during the first hours of exposure. The excess in malonildialdehyde following exposure to WHWW confirmed that oxidative stress severely damaged maize roots. Membrane alteration caused a decrease in NADPH oxidase activity along with DNA damage as confirmed by DNA laddering. The DNA instability was also assessed through sequence-related amplified polymorphism assay, thus suggesting the danger of walnut processing by-product and focusing the attention on the necessity of an efficient treatment of WHWW.
利用核桃收获后加工过程中的副产品核桃壳洗涤水（WHWW）分析其对玉米的化感胁迫，该副产品具有很强的化感潜力和植物毒性效应。WHWW诱导了玉米幼苗根系的氧化损伤和细胞程序性死亡。处理诱导了活性氧爆发，过氧化氢含量过量。在暴露的最初几个小时内，过氧化氢酶的酶活性大幅增加。暴露于WHWW后丙二醛过量，证实氧化应激严重损害了玉米根系。膜改变导致NADPH氧化酶活性降低以及DNA损伤，DNA梯状条带证实了这一点。还通过序列相关扩增多态性分析评估了DNA不稳定性，从而表明核桃加工副产品的危险性，并将注意力集中在有效处理WHWW的必要性上。
