草酸盐对分离的菠菜叶绿体中草酸盐形成和光合作用的影响。
Effect of glycidate on glycolate formation and photosynthesis in isolated spinach chloroplasts.
机构信息
Central Research and Development Department, E. I. du Pont de Nemours & Company, Experimental Station, Wilmington, Delaware 19898.
出版信息
Plant Physiol. 1976 Feb;57(2):237-40. doi: 10.1104/pp.57.2.237.
Glycidate (2,3-epoxypropionate) increased CO(2) photoassimilation in intact spinach (Spinacia oleracea L.) chloroplasts in the presence of various inhibitors of photosynthesis, including O(2), arsenite, azide, iodo-acetamide, and carbonylcyanide 3-chlorophenylhydrazone. Although the mechanism by which glycidate enhances photosynthesis is obscure, the stimulatory effect cannot be ascribed to either an inhibition of glycolate formation, a specific interaction with the O(2) inhibition of photosynthesis, or a direct effect on the ribulose 1,5-bisphosphate carboxylase (EC 4.1.1.39) reaction. The lack of a differential effect of glycidate on photosynthesis and glycolate formation in the isolated chloroplast was confirmed in whole leaf studies by the CO(2) compensation concentration assay. These results are at variance with the report that glycidate stimulates net photosynthesis in tobacco leaf disks by irreversibly inhibiting glycolate formation and thus photorespiration (Zelitch, I., 1974, Arch. Biochem. Biophys. 163: 367-377).
环氧丙酸盐（2,3-环氧丙酸酯）在存在各种光合作用抑制剂的情况下，包括 O₂、亚砷酸盐、叠氮化物、碘乙酰胺和羰基氰化物 3-氯苯腙，增加了完整菠菜（Spinacia oleracea L.）叶绿体的 CO₂光同化。虽然环氧丙酸盐增强光合作用的机制尚不清楚，但这种刺激作用不能归因于甘氨酸形成的抑制、与光合作用 O₂抑制的特定相互作用、或直接作用于核酮糖 1,5-二磷酸羧化酶（EC 4.1.1.39）反应。通过 CO₂补偿浓度测定，在整个叶片研究中证实了环氧丙酸盐在分离的叶绿体中对光合作用和甘氨酸形成没有差异影响。这些结果与环氧丙酸盐通过不可逆地抑制甘氨酸形成从而刺激烟草原生质体中净光合作用的报告不一致（Zelitch，I.，1974，Arch Biochem Biophys 163：367-377）。
Zotero 插件