Departments of Entomology and Biology, Pesticide Research Laboratory and Graduate Study Center, The Pennsylvania State University, University Park, Pennsylvania 16802.
Plant Physiol. 1976 Nov;58(5):666-9. doi: 10.1104/pp.58.5.666.
The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were aspartic and glutamic acid (minor ones) as well as glycine, alanine, and valine (major ones). The last four are being reported for the first time as metabolites of IAA. These conjugates were identified through their chromatographic properties, hydrolysis products, and their mass spectra. The possible significance of these amino acid conjugates is discussed.
我们研究了标记吲哚-3-乙酸（IAA-2-(14)C）在三叶木通冠瘿组织中的代谢。经过 48 小时的孵育，组织吸收了 85%到 90%的供应 IAA，其中约 45%与五种氨基酸结合。发现的结合物为天冬氨酸和谷氨酸（少量）以及甘氨酸、丙氨酸和缬氨酸（主要）。后四种是 IAA 代谢物的首次报道。这些结合物是通过它们的色谱性质、水解产物和质谱来鉴定的。讨论了这些氨基酸结合物的可能意义。
