Laboratoire de Physiologie Cellulaire Végétale, U.A. CNRS No. 576, DRF, CENG and USMG, 85 X, F-38041 Grenoble-Cédex, France.
Plant Physiol. 1987 Aug;84(4):1402-7. doi: 10.1104/pp.84.4.1402.
Protoplasts obtained from sycamore (Acer pseudoplatanus) cell suspensions were found to be highly intact. If the protoplasts were taken up and expelled through a fine nylon mesh, all the protoplasts were ruptured leaving the fragile amyloplasts largely intact. Aconitase hydratase (citrate [isocitrate] hydro-lyase, EC 4.2.1.3) activity of sycamore cells was associated with two protein fractions, one present in the cytosol while the second is of mitochondrial origin. Chromatography on DEAE-trisacryl did not separate the aconitase hydratase isoenzymes. EPR studies established that both isoenzymes exhibited an EPR signal at g = 2.03 once oxidized.
从悬铃木(Acer pseudoplatanus)细胞悬浮液中获得的原生质体高度完整。如果原生质体被吸收并通过细尼龙网挤出,所有的原生质体都会破裂,留下脆弱的淀粉体基本完整。悬铃木细胞的柠檬酸合酶水合酶(柠檬酸[异柠檬酸]水解酶,EC 4.2.1.3)活性与两种蛋白质组分相关,一种存在于细胞质中,另一种来自线粒体。在 DEAE-三丙烯酰上的层析没有分离出柠檬酸合酶同工酶。EPR 研究表明,两种同工酶在氧化后都表现出 g = 2.03 的 EPR 信号。