Travis R L, Woods W G
U.S. Borax Research Corporation, 412 Crescent Way, Anaheim, California 92801.
Plant Physiol. 1977 Jul;60(1):54-7. doi: 10.1104/pp.60.1.54.
The effect of dinitramine, a selective herbicide, on the plasma membrane of the soybean (Glycine max L.) root was studied. Used as marker systems to observe the herbicide effect were two plasma-membrane-specific enzymes, pH 6.5 ATPase and glucan synthetase.The activity of pH 6.5 ATPase decreased significantly in membrane vesicles prepared from roots harvested 15 minutes after treatment with dinitramine. Maximum inhibition occurred in roots harvested 2 hours after treatment. Glucan synthetase activity decreased similarly within 2 hours of treatment.Membrane permeability to (86)Rb was rapidly increased by dinitramine.The activity of pH 6.5 ATPase returned to the control level within 8 hours of treatment with dinitramine.These results show dinitramine's initial site of action to be the plasma membrane, producing an over-all reduction in membrane function through inactivation of membrane-associated proteins.
研究了选择性除草剂二硝胺对大豆(Glycine max L.)根细胞质膜的影响。作为观察除草剂效果的标记系统,使用了两种质膜特异性酶,即pH 6.5 ATP酶和葡聚糖合成酶。在用二硝胺处理15分钟后收获的根制备的膜泡中,pH 6.5 ATP酶的活性显著降低。处理2小时后收获的根中出现最大抑制作用。处理2小时内,葡聚糖合成酶活性也有类似下降。二硝胺使膜对(86)Rb的通透性迅速增加。用二硝胺处理8小时内,pH 6.5 ATP酶的活性恢复到对照水平。这些结果表明,二硝胺的初始作用位点是质膜,通过使膜相关蛋白失活导致膜功能全面降低。