Rolleston F S
Biochem J. 1972 Sep;129(3):721-31. doi: 10.1042/bj1290721.
The binding of ribosomes and ribosomal subunits to endoplasmic reticulum preparations of mouse liver was studied. (1) Membranes prepared from rough endoplasmic reticulum by preincubation with 0.5m-KCl and puromycin bound 60-80% of added 60S subunits and 10-15% of added 40S subunits. Membranes prepared with pyrophosphate and citrate showed less clear specificity for 60S subunits particularly when assayed at low ionic strengths. (2) Ribosomal 40S subunits bound efficiently to membranes only in the presence of 60S subunits. The reconstituted membrane-60S subunit-40S subunit complex was active in synthesis of peptide bonds. (3) No differences in binding to membranes were seen between subunits derived from free and from membrane-bound ribosomes. (4) It is concluded that the binding of ribosomes to membranes does not require that they be translating a messenger RNA, and that the mechanism whereby bound and free ribosomes synthesize different groups of proteins does not depend on two groups of ribosomes that differ in their ability to bind to endoplasmic reticulum.
对核糖体及核糖体亚基与小鼠肝脏内质网制剂的结合进行了研究。(1) 通过与0.5m - KCl和嘌呤霉素预孵育从糙面内质网制备的膜结合了60% - 80%添加的60S亚基和10% - 15%添加的40S亚基。用焦磷酸和柠檬酸盐制备的膜对60S亚基的特异性不太明显，尤其是在低离子强度下检测时。(2) 核糖体40S亚基仅在有60S亚基存在时才能有效地与膜结合。重构的膜 - 60S亚基 - 40S亚基复合物在肽键合成中具有活性。(3) 来自游离核糖体和膜结合核糖体的亚基在与膜的结合上没有差异。(4) 得出的结论是，核糖体与膜的结合并不要求它们正在翻译信使RNA，并且结合核糖体和游离核糖体合成不同组蛋白质的机制并不取决于两组核糖体在结合内质网能力上的差异。
