Fisher J D, Hansen D, Hodges T K
Plant Physiol. 1970 Dec;46(6):812-4. doi: 10.1104/pp.46.6.812.
The energy-dependent influx of Rb(+) into excised roots of corn, wheat, and barley has been determined and compared to the Rb(+)-stimulated ATPase activity of membrane fractions obtained from root homogenates of these species. The external Rb(+) concentrations studied were in the range of 1 to 50 mm. The ratio of Rb(+) influx/Rb(+)-stimulated ATPase was approximately 0.85 and was nearly constant for all the species and Rb(+) concentrations studied. The correlation coefficient for Rb(+) influx versus Rb(+)-activated ATPase was 0.94. The results support the concept that ATP is the energy source for ion transport in roots and that an ATPase participates in the energy transduction process involved in energy-dependent ion transport.
已测定铷离子(Rb(+))在能量依赖下流入玉米、小麦和大麦离体根的情况,并将其与从这些物种根匀浆获得的膜组分中铷离子刺激的ATP酶活性进行了比较。所研究的外部铷离子浓度范围为1至50毫米。铷离子流入量与铷离子刺激的ATP酶的比率约为0.85,并且在所研究的所有物种和铷离子浓度下几乎保持恒定。铷离子流入量与铷离子激活的ATP酶的相关系数为0.94。这些结果支持了以下概念:ATP是根中离子运输的能量来源,并且ATP酶参与了能量依赖型离子运输所涉及的能量转导过程。