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相似文献

1
On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity.关于大肠杆菌细胞分裂的过程。V. 缺乏DNA聚合酶活性的大肠杆菌DNA温度敏感突变体对脱氧核苷三磷酸的掺入
Proc Natl Acad Sci U S A. 1970 Oct;67(2):773-8. doi: 10.1073/pnas.67.2.773.
2
Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.在缺乏DNA聚合酶I的甲苯处理的大肠杆菌中紫外线刺激的DNA合成
Proc Natl Acad Sci U S A. 1973 Jan;70(1):129-33. doi: 10.1073/pnas.70.1.129.
3
Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication.在脱氧核糖核酸复制方面存在缺陷的大肠杆菌K - 12冷敏感突变体的特征
J Bacteriol. 1975 Jan;121(1):99-107. doi: 10.1128/jb.121.1.99-107.1975.
4
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
5
Temperature sensitive mutant of the DNA replication system in Escherichia coli.大肠杆菌中DNA复制系统的温度敏感突变体。
Nature. 1970 Dec 19;228(5277):1157-60. doi: 10.1038/2281157a0.
6
Replication of the Escherichia coli chromosome with a soluble enzyme system.用可溶性酶系统进行大肠杆菌染色体的复制。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3189-93. doi: 10.1073/pnas.71.8.3189.
7
The effect of deoxynucleoside 3'-triphosphates on Escherichia coli DNA polymerase.
Biochim Biophys Acta. 1970 May 21;209(1):250-2. doi: 10.1016/0005-2787(70)90680-5.
8
Further studies on mutants of Escherichia coli with growth selectively affected by fatty acids.
Biochimie. 1974;56(5):729-39. doi: 10.1016/s0300-9084(74)80044-1.
9
Replicative deoixyribonucleic acid synthesis in a system diffusible for macromolecules.在一个对大分子可扩散的系统中进行复制性脱氧核糖核酸合成。
J Biol Chem. 1972 Oct 10;247(19):6031-8.
10
The mechanism of replication of phi X174 single-stranded DNA. VI. Requirements for ribonucleoside triphosphates and DNA polymerase 3.φX174单链DNA的复制机制。VI. 核糖核苷三磷酸和DNA聚合酶3的需求
Can J Biochem. 1973 Dec;51(12):1588-97. doi: 10.1139/o73-214.

引用本文的文献

1
Kinetics of toluene-induced leakage of low molecular weight solutes from excised sorghum tissues.从离体高粱组织中甲苯诱导的低分子量溶质泄漏的动力学。
Plant Physiol. 1981 Dec;68(6):1433-8. doi: 10.1104/pp.68.6.1433.
2
Induction of "pore" formation in plant cell membranes by toluene.甲苯对植物细胞膜“孔”形成的诱导作用。
Plant Physiol. 1978 Feb;61(2):213-7. doi: 10.1104/pp.61.2.213.
3
The gyrB gene product functions in both initiation and chain polymerization of Escherichia coli chromosome replication: suppression of the initiation deficiency in gyrB-ts mutants by a class of rpoB mutations.gyrB基因产物在大肠杆菌染色体复制的起始和链聚合过程中均发挥作用：一类rpoB突变对gyrB温度敏感型突变体起始缺陷的抑制作用。
Mol Gen Genet. 1983;191(2):282-7. doi: 10.1007/BF00334827.
4
Physiological properties of cold-sensitive suppressor mutations of a temperature-sensitive dnaZ mutant of Escherichia coli.大肠杆菌温度敏感型dnaZ突变体的冷敏感抑制突变的生理特性
J Bacteriol. 1983 Jan;153(1):66-75. doi: 10.1128/jb.153.1.66-75.1983.
5
Adenosine triphosphate-dependent synthesis of biologically active DNA by azide-poisoned bacteria.叠氮化物中毒细菌对生物活性DNA的三磷酸腺苷依赖性合成。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1296-300. doi: 10.1073/pnas.68.6.1296.
6
Ribonucleic acid synthesis in bacteria treated with toluene.用甲苯处理的细菌中的核糖核酸合成
J Bacteriol. 1971 Aug;107(2):585-8. doi: 10.1128/jb.107.2.585-588.1971.
7
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
8
Toluene-treated Escherichia coli replicate only that DNA which was about to be replicated in vivo.经甲苯处理的大肠杆菌仅复制那些在体内即将被复制的DNA。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2124-6. doi: 10.1073/pnas.68.9.2124.
9
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
10
Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide.叠氮化物对鼠伤寒沙门氏菌DNA合成及细胞分裂的抑制作用。
Mol Gen Genet. 1974;135(4):339-48. doi: 10.1007/BF00271148.

本文引用的文献

1
Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.脱氧核糖核酸的酶促合成。I. 底物的制备及大肠杆菌中一种酶的部分纯化。
J Biol Chem. 1958 Jul;233(1):163-70.
2
[Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].[大肠杆菌K12的热敏突变体。I. 分离与快速鉴定]
Ann Inst Pasteur (Paris). 1966 Apr;110(4):465-86.
3
Enzymatic synthesis of deoxyribonucleic acid. XXI. Utilization of deoxyribonucleoside triphosphates by Escherichia coli cells.脱氧核糖核酸的酶促合成。 XXI. 大肠杆菌细胞对脱氧核糖核苷三磷酸的利用。
J Biol Chem. 1966 Nov 25;241(22):5419-27.
4
On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria.关于大肠杆菌细胞分裂的过程。一、不对称细胞分裂及无脱氧核糖核酸细菌的产生
J Mol Biol. 1968 Jul 14;35(1):175-92. doi: 10.1016/s0022-2836(68)80046-4.
5
The DNA replicating capacity of isolated E. coli cell wall-membrane complexes.分离出的大肠杆菌细胞壁 - 膜复合物的DNA复制能力。
Nature. 1970 May 23;226(5247):713-7. doi: 10.1038/226713a0.
6
DNA synthesis in vitro.体外DNA合成
Nature. 1970 May 23;226(5247):711-3. doi: 10.1038/226711a0.
7
Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase.对一株具有影响DNA聚合酶突变的大肠杆菌菌株进行基因分析。
Nature. 1969 Dec 20;224(5225):1166-8. doi: 10.1038/2241166a0.
8
Isolation of an E. coli strain with a mutation affecting DNA polymerase.分离出一株具有影响DNA聚合酶突变的大肠杆菌菌株。
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
9
Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.在DNA合成和细胞分裂过程中受到影响的大肠杆菌热敏突变体。
Cold Spring Harb Symp Quant Biol. 1968;33:677-93. doi: 10.1101/sqb.1968.033.01.077.
10
Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA.两个具有温度敏感型DNA合成特性的细菌突变体的表征
Genetics. 1968 Sep;60(1):1-17. doi: 10.1093/genetics/60.1.1.

关于大肠杆菌细胞分裂的过程。V. 缺乏DNA聚合酶活性的大肠杆菌DNA温度敏感突变体对脱氧核苷三磷酸的掺入

On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity.

作者信息

Mordoh J, Hirota Y, Jacob F

出版信息

Proc Natl Acad Sci U S A. 1970 Oct;67(2):773-8. doi: 10.1073/pnas.67.2.773.

DOI:10.1073/pnas.67.2.773
PMID:4943181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283272/
Abstract

Double mutants of Escherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes DNA polymerase activity. Incorporation of labeled deoxynucleoside triphosphates was studied in these mutants using toluene-treated cells. This incorporation was found to be exactly correlated with the capacity of the mutants to synthesize DNA in vivo.

摘要

通过将影响DNA起始（DnaA）或延伸（DnaB）的DNA温度敏感突变与Pol A1突变相结合，构建了大肠杆菌的双突变体，Pol A1突变消除了DNA聚合酶活性。使用甲苯处理的细胞研究了这些突变体中标记的脱氧核苷三磷酸的掺入情况。发现这种掺入与突变体在体内合成DNA的能力完全相关。