关于大肠杆菌细胞分裂的过程。V. 缺乏DNA聚合酶活性的大肠杆菌DNA温度敏感突变体对脱氧核苷三磷酸的掺入
On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity.
作者信息
Mordoh J, Hirota Y, Jacob F
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):773-8. doi: 10.1073/pnas.67.2.773.
Abstract
Double mutants of Escherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes DNA polymerase activity. Incorporation of labeled deoxynucleoside triphosphates was studied in these mutants using toluene-treated cells. This incorporation was found to be exactly correlated with the capacity of the mutants to synthesize DNA in vivo.
摘要
通过将影响DNA起始（DnaA）或延伸（DnaB）的DNA温度敏感突变与Pol A1突变相结合，构建了大肠杆菌的双突变体，Pol A1突变消除了DNA聚合酶活性。使用甲苯处理的细胞研究了这些突变体中标记的脱氧核苷三磷酸的掺入情况。发现这种掺入与突变体在体内合成DNA的能力完全相关。
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