光调节酶活性：通过还原激活光效应介体和通过巯基-二硫键交换调节酶活性。
Light modulation of enzyme activity: activation of the light effect mediators by reduction and modulation of enzyme activity by thiol-disulfide exchange.
机构信息
Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
出版信息
Plant Physiol. 1978 Apr;61(4):601-5. doi: 10.1104/pp.61.4.601.
Abstract
Light and dark modulation experiments with pea (Pisum sativum L.) chloroplast stromal fractions pretreated with dithiothreitol (to reduce protein disulfide bonds) or with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) (to block sulfhydryl groups) suggest that light modulation involves thiol-disulfide exchange on the modulatable stromal enzyme protein. Light-dependent reduction of DTNB involves a photosynthetic electron transport chain component located on the reducing side of photosystem I prior to ferredoxin; DTNB may be acting as a light effect mediator substitute. The thylakoid-bound light effect mediator system, then, in its light-activated reduced form probably catalyzes thiol-disulfide exchange reactions on stromal enzymes.
摘要
用二硫苏糖醇（还原蛋白中二硫键）或 5,5'-二硫代双（2-硝基苯甲酸）（DTNB）（封闭巯基）预处理的豌豆（Pisum sativum L.）叶绿体基质部分进行的光暗调节实验表明，光调节涉及可调节基质酶蛋白上的巯基-二硫键交换。光依赖性 DTNB 的还原涉及位于铁氧还蛋白之前的光系统 I 的还原侧的光合电子传递链组分；DTNB 可能充当光效应介体替代物。因此，类囊体结合的光效应介体系统在其光激活的还原形式下可能催化基质酶上的巯基-二硫键交换反应。
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