Laboratory of Plant Hardiness, Department of Horticulture, University of Minnesota, St. Paul, Minnesota 55101.
Plant Physiol. 1978 Dec;62(6):894-8. doi: 10.1104/pp.62.6.894.
Stem tissues of red-osier dogwood (Cornus stolonifera Michx.) acclimated from -3 C to -40 or -50 C in 8 to 10 weeks under a short photoperiod (9 hours) and controlled temperature conditions. During the summer months plants did not acclimate as well as at other times. The sequence of day/night temperature regimes which induced maximum acclimation was 20/15 C for 5 to 6 weeks; 15/5 C for 2 to 3 weeks; 15/5 C plus 1 hour of frost per day for 1 week. The duration of exposure to each temperature regime influenced the rate and intensity of frost-induced acclimation. Less than 5 weeks of warm temperature preconditioning at 20/15 C reduced subsequent frost-induced acclimation. The inductive influence of frost on cold acclimation was additive over 5 days of repeated exposure, but its effects after the first exposure(s) were not immediate-requiring 1 to 4 days of 15/5 C following the frost treatments for the expression of the frost-induced acclimation to be manifest. There was a 75% increase in rRNA following 3 days of frost exposure and plants in an O(2)-free atmosphere during frost exposure failed to acclimate. The results suggest that seasonal acclimation behavior was due to endogenous rhythms rather than developmental stage, and that the frost-induced phase of acclimation involves aerobic metabolic processes.
红瑞木（Cornus stolonifera Michx.）茎组织在 9 小时短光照和受控温度条件下，经过 8 至 10 周从-3°C 驯化至-40 或-50°C。在夏季，植物的驯化效果不如其他时间好。诱导最大驯化的昼夜温度制度的顺序为 20/15°C 持续 5 至 6 周；15/5°C 持续 2 至 3 周；15/5°C 加每天 1 小时霜持续 1 周。暴露于每个温度制度的持续时间影响霜诱导驯化的速度和强度。少于 5 周的 20/15°C 温暖温度预处理会降低随后的霜诱导驯化。霜对冷驯化的诱导影响在重复暴露 5 天的情况下是累加的，但在第一次暴露后的效果不是立即显现的，在霜处理后需要 1 至 4 天的 15/5°C，才能表现出霜诱导的驯化。经过 3 天的霜暴露后，rRNA 增加了 75%，而在霜暴露期间处于无氧气氛中的植物无法驯化。结果表明，季节性驯化行为是由于内源性节律而不是发育阶段，并且驯化的霜诱导阶段涉及有氧代谢过程。
