Reger B J, Yates I E
Richard B. Russell Research Center, Science and Education Administration, United States Department of Agriculture, Athens, Georgia 30604.
Plant Physiol. 1979 Jan;63(1):209-12. doi: 10.1104/pp.63.1.209.
Arundinella hirta L. is a C(4) plant having an unusual C(4) leaf anatomy. Besides mesophyll and bundle sheath cells, A. hirta leaves have specialized parenchyma cells which look morphologically like bundle sheath cells but which lack vascular connections and are located between veins, running parallel to them. Activities of phosphoenolpyruvate and ribulose-1,5-bisphosphate carboxylases and phosphoenolpyruvate carboxykinase, NADP-and NAD-malic enzymes were determined for whole leaf extracts and isolated mesophyll protoplasts, specialized parenchyma cells, and bundle sheath cells. The data indicate that A. hirta is a NADP-malic enzyme type C(4) species. In addition, specialized parenchyma cells and bundle sheath cells are enzymatically alike. Compartmentation of enzymes followed the C(4) pattern with phosphoenolpyruvate carboxylase being restricted to mesophyll cells while ribulose-1,5-bisphosphate carboxylase and decarboxylating enzymes were restricted to bundle sheath and specialized parenchyma cells.
野古草是一种具有独特C4叶解剖结构的C4植物。除了叶肉细胞和维管束鞘细胞外，野古草叶片还有特化的薄壁细胞，这些细胞在形态上类似于维管束鞘细胞，但缺乏维管连接，位于叶脉之间并与叶脉平行排列。测定了全叶提取物、分离的叶肉原生质体、特化薄壁细胞和维管束鞘细胞中磷酸烯醇丙酮酸羧化酶、核酮糖-1,5-二磷酸羧化酶、磷酸烯醇丙酮酸羧激酶、NADP-和NAD-苹果酸酶的活性。数据表明野古草是一种NADP-苹果酸酶型C4植物。此外，特化薄壁细胞和维管束鞘细胞在酶学上相似。酶的区室化遵循C4模式，磷酸烯醇丙酮酸羧化酶局限于叶肉细胞，而核酮糖-1,5-二磷酸羧化酶和脱羧酶局限于维管束鞘细胞和特化薄壁细胞。
