发芽蓖麻胚乳中的三磷酸柠檬酸裂解酶：定位和一些性质。
ATP Citrate Lyase from Germinating Castor Bean Endosperm: Localization and some Properties.
机构信息
Thimann Laboratories, University of California, Santa Cruz, California 95064.
出版信息
Plant Physiol. 1979 Apr;63(4):687-91. doi: 10.1104/pp.63.4.687.
Abstract
ATP citrate lyase (EC 4.1.3.8) has been found in crude extracts from endosperm tissue of germinating castor bean and shows its maximum activity in 4- to 5-day-old seedlings. A strict requirement for coenzyme A and adenosine 5'-triphosphate was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. (-)-Hydroxycitrate is a potent inhibitor. The molecular weight is about 400,000. The adenosine 5'-triphosphate citrate lyase is localized in the plastids, where it possibly plays a role in providing acetyl coenzyme A for lipid biosynthesis.
摘要
三磷酸柠檬酸裂合酶（EC 4.1.3.8）在发芽蓖麻胚乳组织的粗提物中被发现，并在 4 至 5 日龄的幼苗中表现出最大活性。证明该酶对辅酶 A 和腺苷 5'-三磷酸有严格的要求。反应的最适 pH 值约为 7.5。不稳定的酶可以通过冷冻和添加柠檬酸和甘油来稳定。（-）-羟基柠檬酸是一种有效的抑制剂。分子量约为 400,000。三磷酸柠檬酸裂合酶定位于质体中，可能在为脂质生物合成提供乙酰辅酶 A 方面发挥作用。
相似文献
1
ATP Citrate Lyase from Germinating Castor Bean Endosperm: Localization and some Properties.发芽蓖麻胚乳中的三磷酸柠檬酸裂解酶：定位和一些性质。
Plant Physiol. 1979 Apr;63(4):687-91. doi: 10.1104/pp.63.4.687.
2
Control of gluconeogenesis by isocitrate lyase in endosperm of germinating castor bean seedlings.异柠檬酸裂解酶对蓖麻籽萌发幼苗胚乳中糖异生作用的调控
Plant J. 1999 Aug;19(4):423-31. doi: 10.1046/j.1365-313x.1999.00533.x.
3
Proteinases and enzyme stability in crude extracts of castor bean endosperm.蓖麻籽胚乳粗提物中的蛋白酶与酶稳定性
Plant Physiol. 1981 Mar;67(3):499-502. doi: 10.1104/pp.67.3.499.
4
Molecular and immunological characterization of plastid and cytosolic pyruvate kinase isozymes from castor-oil-plant endosperm and leaf.蓖麻籽胚乳和叶片中质体和胞质丙酮酸激酶同工酶的分子与免疫学特性
Eur J Biochem. 1989 May 1;181(2):443-51. doi: 10.1111/j.1432-1033.1989.tb14745.x.
5
Subcellular distribution of gluconeogenetic enzymes in germinating castor bean endosperm.蓖麻籽萌发胚乳中糖异生酶的亚细胞分布
Plant Physiol. 1979 Jul;64(1):31-7. doi: 10.1104/pp.64.1.31.
6
Coenzyme A is required for rat liver fatty acid synthetase activity.辅酶A是大鼠肝脏脂肪酸合成酶活性所必需的。
J Biol Chem. 1980 Feb 25;255(4):1388-92.
7
Changes in stability of isocitrate dehydrogenase (NADP+) during germination of castor bean seeds.蓖麻籽种子萌发过程中异柠檬酸脱氢酶（NADP⁺）稳定性的变化。
Biochim Biophys Acta. 1980 Jul 3;630(3):352-60. doi: 10.1016/0304-4165(80)90283-4.
8
Fatty Acid synthesis in endosperm of young castor bean seedlings.蓖麻幼胚乳中脂肪酸的合成。
Plant Physiol. 1978 Aug;62(2):173-8. doi: 10.1104/pp.62.2.173.
9
Localization and properties of ribulose diphosphate carboxylase from castor bean endosperm.蓖麻籽胚乳中二磷酸核酮糖羧化酶的定位与特性
Plant Physiol. 1975 Feb;55(2):226-30. doi: 10.1104/pp.55.2.226.
10
Immunological and biochemical studies on isozymes of malate dehydrogenase and citrate synthetase in castor bean glyoxysomes.蓖麻乙醛酸循环体中苹果酸脱氢酶和柠檬酸合成酶同工酶的免疫学和生物化学研究。
Plant Physiol. 1974 Sep;54(3):364-7. doi: 10.1104/pp.54.3.364.
引用本文的文献
1
The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves.水稻斑马叶3（z3）突变破坏了柠檬酸分布，并在成熟叶片中产生横向深绿色/绿色斑纹。
Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.
2
The role of pyruvate hub enzymes in supplying carbon precursors for fatty acid synthesis in photosynthetic microalgae.丙酮酸枢纽酶在为光合微藻脂肪酸合成提供碳前体中的作用。
Photosynth Res. 2015 Sep;125(3):407-22. doi: 10.1007/s11120-015-0136-7. Epub 2015 Apr 7.
3
Intracellular location of ATP citrate lyase in leaves of Pisum sativum L.豌豆叶片中三磷酸柠檬酸裂解酶的细胞内定位
Planta. 1985 Feb;163(2):290-4. doi: 10.1007/BF00393520.
4
Coimmunopurification of phosphorylated bacterial- and plant-type phosphoenolpyruvate carboxylases with the plastidial pyruvate dehydrogenase complex from developing castor oil seeds.从发育中的蓖麻籽中，磷酸化的细菌型和植物型磷酸烯醇式丙酮酸羧化酶与质体丙酮酸脱氢酶复合体的共免疫纯化。
Plant Physiol. 2008 Mar;146(3):1346-57. doi: 10.1104/pp.107.110361. Epub 2008 Jan 9.
5
Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism.纯化花椰菜芽质体中脂质合成和碳水化合物代谢的酶活性。
Plant Physiol. 1985 Oct;79(2):458-67. doi: 10.1104/pp.79.2.458.
6
Isoenzymes of sugar phosphate metabolism in endosperm of germinating castor beans.萌发蓖麻籽胚乳中磷酸糖代谢的同工酶
Plant Physiol. 1981 Jun;67(6):1255-8. doi: 10.1104/pp.67.6.1255.
7
Role of malate synthase in citric Acid synthesis by maturing cotton embryos: a proposal.苹果酸合酶在成熟棉胚柠檬酸合成中的作用：一种假说。
Plant Physiol. 1981 May;67(5):875-81. doi: 10.1104/pp.67.5.875.
8
Molecular characterization of a heteromeric ATP-citrate lyase that generates cytosolic acetyl-coenzyme A in Arabidopsis.拟南芥中一种产生胞质乙酰辅酶A的异源三聚体ATP-柠檬酸裂解酶的分子特征
Plant Physiol. 2002 Oct;130(2):740-56. doi: 10.1104/pp.008110.
9
The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds.丙酮酸脱氢酶和乙酰辅酶A合成酶在拟南芥种子发育过程中脂肪酸合成中的作用。
Plant Physiol. 2000 Jun;123(2):497-508. doi: 10.1104/pp.123.2.497.
10
Genetic enhancement of fatty acid synthesis by targeting rat liver ATP:citrate lyase into plastids of tobacco.通过将大鼠肝脏ATP：柠檬酸裂解酶靶向烟草质体来进行脂肪酸合成的遗传增强。
Plant Physiol. 2000 Apr;122(4):1231-8. doi: 10.1104/pp.122.4.1231.
本文引用的文献
1
Fatty Acid synthesis in endosperm of young castor bean seedlings.蓖麻幼胚乳中脂肪酸的合成。
Plant Physiol. 1978 Aug;62(2):173-8. doi: 10.1104/pp.62.2.173.
2
Isolation of intact plastids from protoplasts from castor bean endosperm.从蓖麻胚乳原生质体中分离完整的质体。
Plant Physiol. 1978 Jul;62(1):40-3. doi: 10.1104/pp.62.1.40.
3
Enzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinus communis L.蓖麻发育中胚乳前质体中糖酵解途径和戊糖磷酸途径的酶
Plant Physiol. 1977 Jun;59(6):1128-32. doi: 10.1104/pp.59.6.1128.
4
Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids.高等植物线粒体和前质体中的丙酮酸脱氢酶复合物。
Plant Physiol. 1977 May;59(5):842-8. doi: 10.1104/pp.59.5.842.
5
Localization and properties of ribulose diphosphate carboxylase from castor bean endosperm.蓖麻籽胚乳中二磷酸核酮糖羧化酶的定位与特性
Plant Physiol. 1975 Feb;55(2):226-30. doi: 10.1104/pp.55.2.226.
6
Citrate cleavage enzymes from developing soybean cotyledons: incorporation of citrate carbon into Fatty acids.发育中的大豆子叶中的柠檬酸裂解酶：柠檬酸碳掺入脂肪酸中。
Plant Physiol. 1975 Jan;55(1):69-72. doi: 10.1104/pp.55.1.69.
7
Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.富马酸和顺乌头酸酶促形成的分光光度测量。
Biochim Biophys Acta. 1950 Jan;4(1-3):211-4. doi: 10.1016/0006-3002(50)90026-6.
8
The citrate cleavage enzyme. I. Distribution and purification.柠檬酸裂解酶。I. 分布与纯化。
J Biol Chem. 1959 Oct;234:2544-7.
9
Mitochondria and glyoxysomes from castor bean endosperm. Enzyme constitutents and catalytic capacity.蓖麻籽胚乳中的线粒体和乙醛酸循环体。酶成分及催化能力。
J Biol Chem. 1969 Jul 10;244(13):3507-13.
10
Influence of sucrose on protein determination by the Lowry procedure.蔗糖对采用洛瑞法测定蛋白质的影响。
Anal Biochem. 1968 Aug;24(2):337-9. doi: 10.1016/0003-2697(68)90187-5.
Zotero 插件