Agricultural Research Council Research Group, School of Chemistry, The University, BS8 1TS, Bristol, UK.
Planta. 1983 Nov;159(5):454-68. doi: 10.1007/BF00392082.
Gibberellin A20 (GA20), GA29 and GA29-catabolite were quantified in cotyledons, embryonic axes, and testas of Pisum sativum cv. Progress No. 9 throughout the final stages of seed maturation and during germination. Stable isotope-labelled GAs were used as internal standards in conjunction with combined gas chromatography-mass spectrometry. Gibberellin A20 and GA29 were mainly located in the cotyledons of maturing seeds, and GA29-catabolite was predominantly located in the testa. Stable isotope- and radio-labelled GA20 and GA29 were fed to both intact seeds developing in vivo, and to isolated seed parts cultured in vitro. The combined results of in-vivo and in-vitro feeds indicated that GA20 is metabolised to GA29 in the cotyledons, that GA29 is transported from the cotyledons to the testa, and that GA29 is metabolised to GA29-catabolite in the testa. Although the metabolism of GA20 in the cotyledons and of GA29 in the testa has been shown definitively, the mobility of GA29 has not yet been demonstrated directly. During seed desiccation and germination GA29-catabolite and products arising from it are transferred from the testa into the embryo. There is no evidence of a physiological function for GA29-catabolite in germination or early seedling growth. Use of a growth retardant indicates that seedling growth, but not germination, is dependent on de-novo GA biosynthesis.
在豌豆品种 Progress No.9 种子成熟的最后阶段和萌发过程中，我们定量分析了赤霉素 A20（GA20）、GA29 和 GA29 代谢产物在子叶、胚胎轴和种皮中的含量。我们使用稳定同位素标记的赤霉素作为内标，结合气相色谱-质谱联用技术进行分析。GA20 和 GA29 主要位于成熟种子的子叶中，GA29 代谢产物主要位于种皮中。我们将稳定同位素和放射性标记的 GA20 和 GA29 分别饲喂到体内发育的完整种子和体外培养的种子分离部分。体内和体外饲喂的综合结果表明，GA20 在子叶中代谢为 GA29，GA29 从子叶转运到种皮，GA29 在种皮中代谢为 GA29 代谢产物。虽然已经明确证明了 GA20 在子叶中的代谢和 GA29 在种皮中的代谢，但 GA29 的移动性尚未直接证明。在种子干燥和萌发过程中，GA29 代谢产物及其衍生产物从种皮转移到胚中。GA29 代谢产物在萌发或早期幼苗生长中没有生理功能的证据。使用生长抑制剂表明，幼苗生长（但不是萌发）依赖于从头合成 GA。
