Agricultural Research Council Research Group, School of Chemistry, The University, BS8 1TS, Bristol, UK.
Planta. 1984 Dec;162(6):560-5. doi: 10.1007/BF00399923.
Twenty known gibberellins (GAs) have been identified by combined capillary gas chromatography-mass spectrometry in extracts from less than 10 g fresh weight of maturing seeds of the cucurbit Sechium edule Sw. The GAs are predominantly 3- and-or 13-hydroxylated. This is the first reported identification of non-conjugated 13-hydroxylated GAs in a cucurbit. Gibberellin A8 and gibberellin A8-catabolite are the major GAs in terms of quantity and are largely accumulated in the testa. The catabolites of 2β-hydroxylated GAs are α,β-unsaturated ketones which no longer possess of a γ-lactone. They were hitherto known only in legumes. The presence of GA8-catabolite as a major component of Sechium seeds indicates that the distribution of these GA-catabolites may be more widespread than previously envisaged. The localization of known GAs in maturing seeds of the legume Phaseolus coccineus L. was found to resemble closely that in Sechium. Gibberellin A8, a putative conjugate of GA8 and GA8-catabolite are accumulated in the testa. The localization in the testa of end-products of the GA-biosynthetic pathway, which was first observed in maturing seeds of Pisum sativum, and is now described in Phaseolus and Sechium, may be a general feature of seed development.
在从成熟的葫芦科植物笋瓜（Sechium edule Sw.）种子不到 10 克的鲜重提取物中，通过毛细管气相色谱-质谱联用技术鉴定出了 20 种已知的赤霉素（GA）。GA 主要是 3-和/或 13-羟基化的。这是首次在葫芦科植物中报道非共轭 13-羟基化 GA 的鉴定。就数量而言，赤霉素 A8 和赤霉素 A8 代谢物是主要的 GA，大量积累在种皮中。2β-羟基化 GA 的代谢物是α，β-不饱和酮，不再具有γ-内酯。它们以前只在豆科植物中被发现。GA8 代谢物作为笋瓜种子的主要成分的存在表明，这些 GA 代谢物的分布可能比以前预期的更为广泛。在豆科植物菜豆（Phaseolus coccineus L.）成熟种子中发现的已知 GA 的定位与笋瓜非常相似。赤霉素 A8，GA8 和 GA8 代谢物的一种假定轭合物，积累在种皮中。GA 生物合成途径的终产物在豌豆（Pisum sativum）成熟种子中首次观察到，现在在菜豆和笋瓜中也有描述，其在种皮中的定位可能是种子发育的一个普遍特征。
