Salin M L, Bridges S M
Department of Biochemistry, Mississippi State University, Mississippi State, Mississippi 39762.
Plant Physiol. 1981 Jan;67(1):43-6. doi: 10.1104/pp.67.1.43.
Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN(-) and N(3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segments as did ascorbic acid and hydroquinone. Superoxide dismutase addition resulted in a small diminution in light emission, but mannitol, an OH. scavenger, was without effect. The addition of H(2)O(2) to wounded root segments markedly elevated chemiluminescence in the presence of air as well as under N(2). It is concluded that peroxidases, found abundantly in roots, predominantly contribute to light emission in wounded plant tissue.
大豆(品种为Merrill cv. ;Bragg')的根段和茎段在受到机械损伤后会出现增强的化学发光现象。根发出的光比茎多。化学发光被CN(-)和N(3) (-)减弱,但不受鱼藤酮和抗霉素A的影响。过氧化氢酶与抗坏血酸和对苯二酚一样,能淬灭受伤根段中的化学发光。添加超氧化物歧化酶会使发光略有减弱,但OH.清除剂甘露醇则没有效果。在有空气以及氮气存在的情况下,向受伤根段添加H(2)O(2)会显著提高化学发光。得出的结论是,在根中大量存在的过氧化物酶主要导致受伤植物组织中的发光。