United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701.
Plant Physiol. 1978 Nov;62(5):696-8. doi: 10.1104/pp.62.5.696.
Light production by plants was confirmed by measuring chemiluminescence from root and stem tissue of peas (Pisum sativum), beans (Phaseolus vulgaris), and corn (Zea mays) in a modified scintillation spectrophotometer. Chemiluminescence was inhibited by treating pea roots with boiling ethanol or by placing them in a N(2) gas phase. Chemiluminescence was increased by an O(2) gas phase or by the addition of luminol. NaN(3) and NaCN blocked both in vitro and in vivo chemiluminescence.It is postulated that the source of light is the hydrogen peroxide-peroxidase enzyme system. It is known that this system is responsible for chemiluminescence in leukocytes and it seems likely that a similar system occurs in plants.
采用改良的闪烁分光光度计,通过测量豌豆(Pisum sativum)、菜豆(Phaseolus vulgaris)和玉米(Zea mays)的根和茎组织的化学发光,证实了植物的发光现象。用沸乙醇处理豌豆根或使其处于氮气气相中,可抑制化学发光。向体系中通入氧气或加入鲁米诺,可增强化学发光。NaN3 和 NaCN 均可阻断体外和体内化学发光。据推测,发光的来源是过氧化氢-过氧化物酶酶系统。已知该系统负责白细胞中的化学发光,而且类似的系统似乎也存在于植物中。