赤霉素 A3 对萌发蓖麻胚乳中内质网和乙醛酸体形成的影响。
Effect of gibberellin a(3) on the endoplasmic reticulum and on the formation of glyoxysomes in the endosperm of germinating castor bean.
机构信息
Department of Biology, University of California, Los Angeles, California 90024.
出版信息
Plant Physiol. 1978 Sep;62(3):449-53. doi: 10.1104/pp.62.3.449.
Seedlings of castor bean (Ricinus communis cv. Hale) were exposed to a range of concentrations of gibberellin A(3) (GA(3)). Treatments for 20 hours with GA(3) concentrations of 0.5 muM or higher resulted in increased levels of NADH-cytochrome c reductase, phosphorylcholine glyceride transferase, and malate synthase in endoplasmic reticulum (ER) isolated from endosperm on linear sucrose gradients. GA(3) treatment also resulted in increased RNA associated with ER. Malate synthase and catalase in crude homogenates were enhanced by 1 to 100 muM GA(3) concentrations. Isocitrate lyase, citrate synthase, malate synthase, catalase, and glycolate oxidase in isolated glyoxysomes were enhanced by 60, 20, 18, 40, and 28%, respectively, over controls. Treatment with abscisic acid led to decreased levels of glyoxysomal enzymes and reduced glyoxysomal protein. The effect of GA(3) and abscisic acid on the specific activities of glyoxysomes of different densities suggests that GA(3) influences enzyme levels and glyoxysome assembly.
蓖麻种子（Ricinus communis cv. Hale）幼苗暴露在一系列赤霉素 A（3）（GA（3））浓度下。用 GA（3）处理 20 小时，浓度为 0.5 μM 或更高，导致从胚乳中分离的内质网（ER）中 NADH-细胞色素 c 还原酶、磷酸胆碱甘油酯转移酶和苹果酸合酶的水平增加。GA（3）处理还导致与 ER 相关的 RNA 增加。粗匀浆中的苹果酸合酶和过氧化氢酶分别被 1 到 100 μM GA（3）浓度增强 1 到 100 倍。分离的乙醛酸体中的异柠檬酸裂解酶、柠檬酸合酶、苹果酸合酶、过氧化氢酶和乙醇酸氧化酶分别比对照增强了 60%、20%、18%、40%和 28%。脱落酸处理导致乙醛酸体酶水平降低和乙醛酸体蛋白减少。GA（3）和脱落酸对不同密度乙醛酸体的比活性的影响表明，GA（3）影响酶水平和乙醛酸体的组装。
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