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柑橘幼苗叶片脂肪酸与抗寒锻炼的关系。

Relationships of leaf Fatty acids to cold hardening of citrus seedlings.

作者信息

Nordby H E, Yelenosky G

机构信息

United States Citrus and Subtropical Products Laboratory, Winter Haven, Florida 33880.

出版信息

Plant Physiol. 1982 Jul;70(1):132-5. doi: 10.1104/pp.70.1.132.

DOI:10.1104/pp.70.1.132
PMID:16662433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1067100/
Abstract

Three cultivars of citrus with different sensitivities to freezing temperatures (citron, Citrus medica L.; rough lemon, C. limon Burm. F; sour orange, C. aurantium L.) were cold hardened for 4 weeks. Lipids from leaves of hardened and control seedlings were fractionated and analyzed for fatty acids. The absolute amount of triglycerides and phospholipids increased in the leaves upon hardening. With hardening, total linoleic acid also increased 141% in citron, 210% in rough lemon, and 233% in sour orange. Specific increases in linoleic acid were found in triglycerides, in the four phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol), and in neutral lipids more polar than triglycerides. Trans-3-hexadecenoic acid was found only in phosphatidylglycerol.

摘要

对低温具有不同敏感性的三个柑橘品种(枸橼,Citrus medica L.;粗柠檬,C. limon Burm. F;酸橙,C. aurantium L.)进行了4周的抗寒锻炼。对经过抗寒锻炼和未经锻炼的幼苗叶片中的脂质进行分级分离并分析脂肪酸。抗寒锻炼后,叶片中甘油三酯和磷脂的绝对含量增加。随着抗寒锻炼,枸橼中总亚油酸增加了141%,粗柠檬中增加了210%,酸橙中增加了233%。甘油三酯、四种磷脂(磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰甘油)以及比甘油三酯极性更强的中性脂质中的亚油酸都有特定增加。反式-3-十六碳烯酸仅在磷脂酰甘油中被发现。

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本文引用的文献

1
Cold hardening in citrus stems.柑橘茎的冷驯化。
Plant Physiol. 1975 Oct;56(4):540-3. doi: 10.1104/pp.56.4.540.
2
Lipids in alfalfa leaves in relation to cold hardiness.紫花苜蓿叶片中的脂质与抗寒性的关系。
Plant Physiol. 1970 Jun;45(6):684-6. doi: 10.1104/pp.45.6.684.
3
A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS.一种用于检测薄层色谱图上磷脂的简单、特效喷雾剂。
J Lipid Res. 1964 Jan;5:126-7.
4
Solidification of unsaturated/saturated fatty acid mixtures and its relationship to chilling sensitivity in plants.不饱和/饱和脂肪酸混合物的固化及其与植物冷敏感性的关系。
J Am Oil Chem Soc. 1965 Dec;42(12):1056-8. doi: 10.1007/BF02636905.
5
Studies on membranes in plant cells resistant to extreme freezing. I. Augmentation of phospholipids and membrane substance without changes in unsaturation of fatty acids during hardening of black locust bark.植物细胞中抗极端冷冻的膜的研究。I. 刺槐树皮硬化过程中磷脂和膜物质增加而脂肪酸不饱和度不变。
Cryobiology. 1975 Apr;12(2):144-53. doi: 10.1016/s0011-2240(75)80006-x.