Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, D-3300 Braunschweig, West Germany.
Plant Physiol. 1982 Jul;70(1):74-7. doi: 10.1104/pp.70.1.74.
Studies with purified chloroplasts of Lupinus polyphyllus LINDL. leaflets indicate that the first two enzymes of quinolizidine alkaloid biosynthesis, lysine decarboxylase and 17-oxosparteine synthase, are localized in the chloroplast stroma. Thus, both enzymes share the same subcellular compartment as the biosynthetic pathway of lysine, the precursor of quinolizidine alkaloids. The activity of diaminopimelate decarboxylase, the final enzyme in lysine biosynthesis, is about two to three orders of magnitude higher than that of the enzymes of alkaloid formation.
对羽扇豆小叶的纯化叶绿体进行的研究表明，喹啉生物碱生物合成的前两个酶，赖氨酸脱羧酶和 17-氧代高丝氨酸合酶，定位于叶绿体基质中。因此，这两种酶与赖氨酸生物合成途径共享相同的亚细胞区室，赖氨酸是喹啉生物碱的前体。赖氨酸生物合成的最后一个酶二氨基庚二酸脱羧酶的活性比生物碱形成酶的活性高两到三个数量级。
