Ohnishi Miho, Furutani Riu, Sohtome Takayuki, Suzuki Takeshi, Wada Shinya, Tanaka Soma, Ifuku Kentaro, Ueno Daisei, Miyake Chikahiro
Department of Applied Biological Science, Graduate School for Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Core Research for Environmental Science and Technology (CREST), Japan Science and Technology Agency (JST), 7 Gobancho, Tokyo 102-0076, Japan.
Antioxidants (Basel). 2021 Jun 23;10(7):996. doi: 10.3390/antiox10070996.
In response to decreases in the assimilation efficiency of CO, plants oxidize the reaction center chlorophyll (P700) of photosystem I (PSI) to suppress reactive oxygen species (ROS) production. In hydro-cultured sunflower leaves experiencing essential mineral deficiencies, we analyzed the following parameters that characterize PSI and PSII: (1) the reduction-oxidation states of P700 [Y(I), Y(NA), and Y(ND)]; (2) the relative electron flux in PSII [Y(II)]; (3) the reduction state of the primary electron acceptor in PSII, Q (1 - qL); and (4) the non-photochemical quenching of chlorophyll fluorescence (NPQ). Deficiency treatments for the minerals N, P, Mn, Mg, S, and Zn decreased Y(II) with an increase in the oxidized P700 [Y(ND)], while deficiencies for the minerals K, Fe, Ca, B, and Mo decreased Y(II) without an increase in Y(ND). During the induction of photosynthesis, the above parameters showed specific responses to each mineral. That is, we could diagnose the mineral deficiency and identify which mineral affected the photosynthesis parameters.
为应对二氧化碳同化效率的降低，植物会氧化光系统I（PSI）的反应中心叶绿素（P700）以抑制活性氧（ROS）的产生。在水培向日葵叶片出现必需矿物质缺乏的情况下，我们分析了以下表征PSI和PSII的参数：（1）P700的氧化还原状态[Y(I)、Y(NA)和Y(ND)]；（2）PSII中的相对电子通量[Y(II)]；（3）PSII中初级电子受体Q的还原状态（1 - qL）；以及（4）叶绿素荧光的非光化学猝灭（NPQ）。对矿物质N、P、Mn、Mg、S和Zn的缺乏处理会使Y(II)降低，同时氧化态的P700 [Y(ND)]增加，而对矿物质K、Fe、Ca、B和Mo的缺乏处理会使Y(II)降低，但Y(ND)不会增加。在光合作用诱导过程中，上述参数对每种矿物质都表现出特定的响应。也就是说，我们可以诊断矿物质缺乏情况，并确定哪种矿物质影响了光合作用参数。
