细胞内 pH 值的变化与 Neurospora 的昼夜节律无关。
Changes in Intracellular pH Are Not Correlated with the Circadian Rhythm of Neurospora.
机构信息
Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
出版信息
Plant Physiol. 1983 May;72(1):129-33. doi: 10.1104/pp.72.1.129.
Intracellular pH (pH(i)) was measured during the circadian cycle of Neurospora. Internal pH of Neurospora cultures in liquid medium was assayed by the 5,5-dimethyl-2,4-oxazolidinedione method and gave values for pH(i) which were similar to those previously obtained by other workers using pH-microelectrodes with agar-grown cultures. Cytoplasmic pH changed in liquid medium cultures, but these changes were not related to the circadian clock. Furthermore, treatments which raise or lower pH(i) do not phase-shift the circadian rhythm. These results indicate that pH(i) plays no specific role in regulating the circadian clock of Neurospora.
细胞内 pH 值（pH(i)）在 Neurospora 的昼夜节律周期中进行了测量。通过 5,5-二甲基-2,4-恶唑烷二酮法测定液体培养基中 Neurospora 培养物的内部 pH 值，得到的 pH(i)值与先前使用琼脂生长培养物的 pH 微电极获得的值相似。细胞质 pH 在液体培养基培养物中发生变化，但这些变化与昼夜节律时钟无关。此外，升高或降低 pH(i)值的处理不会使昼夜节律节律发生相移。这些结果表明，pH(i)在调节 Neurospora 的昼夜节律时钟中没有发挥特定作用。
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