Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Plant Physiol. 1983 May;72(1):129-33. doi: 10.1104/pp.72.1.129.
Intracellular pH (pH(i)) was measured during the circadian cycle of Neurospora. Internal pH of Neurospora cultures in liquid medium was assayed by the 5,5-dimethyl-2,4-oxazolidinedione method and gave values for pH(i) which were similar to those previously obtained by other workers using pH-microelectrodes with agar-grown cultures. Cytoplasmic pH changed in liquid medium cultures, but these changes were not related to the circadian clock. Furthermore, treatments which raise or lower pH(i) do not phase-shift the circadian rhythm. These results indicate that pH(i) plays no specific role in regulating the circadian clock of Neurospora.
细胞内 pH 值（pH(i)）在 Neurospora 的昼夜节律周期中进行了测量。通过 5,5-二甲基-2,4-恶唑烷二酮法测定液体培养基中 Neurospora 培养物的内部 pH 值，得到的 pH(i)值与先前使用琼脂生长培养物的 pH 微电极获得的值相似。细胞质 pH 在液体培养基培养物中发生变化，但这些变化与昼夜节律时钟无关。此外，升高或降低 pH(i)值的处理不会使昼夜节律节律发生相移。这些结果表明，pH(i)在调节 Neurospora 的昼夜节律时钟中没有发挥特定作用。
