正常玉米籽粒中结合型淀粉粒结合淀粉合成酶的溶解。
Solubilization of the starch-granule-bound starch synthase of normal maize kernels.
机构信息
Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1983 Sep;73(1):175-8. doi: 10.1104/pp.73.1.175.
Abstract
The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significant activity with UDPglucose.
摘要
从玉米中分离出来的淀粉粒结合型淀粉合酶已经实现了 50%到 84%的回收率。用 DEAE-琼脂糖凝胶对溶解的酶进行层析分离，得到了两个具有活性的级分，它们可以通过对柠檬酸的反应来区分。这两种可溶性同工酶与 UDPglucose 都没有显示出任何显著的活性。
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本文引用的文献
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2
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