Division of Molecular Plant Biology, University of California, Berkeley, California 94720.
Plant Physiol. 1983 Nov;73(3):724-8. doi: 10.1104/pp.73.3.724.
Cultures of the blue-green alga, Aphanocapsa, were grown under iron-limiting conditions and changes in concentration of redox components of the photosynthetic electron transport chain, particularly iron-sulfur centers, were monitored by spectroscopic methods. A moderate iron depletion (1/10 of the normal concentration) had little effect on photosynthetic electron transport reactions and growth. Nevertheless, the amount of membrane-bound non-heme iron decreased sharply, and ferredoxin was nearly totally replaced by a flavin-containing protein, flavodoxin. Severe iron-deficiency (1/100 of the normal concentration) was accompanied by growth inhibition and decreased rates of photosynthetic electron flow. The Photosystem I reaction center was most affected by iron depletion as evidenced by a decrease in the amounts of iron-sulfur centers A, B, and X. However, formation of other redox proteins, even those that do not contain iron, was also inhibited by severe iron deficiency.
在缺铁条件下培养蓝绿藻 Aphanocapsa，并通过光谱方法监测光合作用电子传递链中氧化还原成分（特别是铁硫中心）的浓度变化。适度的铁缺乏（正常浓度的 1/10）对光合作用电子传递反应和生长几乎没有影响。然而，膜结合的非血红素铁的含量急剧下降，铁氧还蛋白几乎完全被含有黄素的蛋白黄素氧还蛋白取代。严重的缺铁（正常浓度的 1/100）伴随着生长抑制和光合作用电子流速率的降低。光系统 I 反应中心受铁缺乏的影响最大，这表现在铁硫中心 A、B 和 X 的含量减少。然而，即使是不含铁的其他氧化还原蛋白的形成也受到严重缺铁的抑制。
