Federal Center for Lipid Research, Institute for Biochemistry and Technology-H. P. Kaufmann-Institute, Piusallee 68, D-4400 Münster, Federal Republic of Germany.
Plant Physiol. 1983 Dec;73(4):929-34. doi: 10.1104/pp.73.4.929.
Cotyledons of developing mustard (Sinapis alba L.) seed have been found to synthesize lipids containing the common plant fatty acids and very long-chain monounsaturated (icosenoic, erucic, and tetracosenic) and saturated (icosanoic, docosanoic, and tetracosanoic) fatty acids from various radioactive precursors. The in vivo pattern of labeling of acyl lipids, either from fatty acids synthesized ;endogenously' from radioactive acetate or malonate, or from radioactive fatty acids added ;exogenously', indicates the involvement of the following pathways in the biosynthesis of triacylglycerols. Palmitic, stearic, and oleic acid, synthesized in the acyl carrier protein-track, are channeled to the Coenzyme A (CoA)-track and converted to triacylglycerols via the glycerol-3-phosphate pathway. Pools of stearoyl-CoA and oleoyl-CoA are elongated to very long-chain saturated and monounsaturated acyl-CoA, respectively. Most of the very long-chain saturated acyl-CoAs acylate preformed diacylglycerols. Very long-chain monounsaturated acyl-CoAs are converted to triacylglycerols, partly via phosphatidic acids and diacylglycerols, and partly by acylation of preformed diacylglycerols.
发育中的芥菜（Sinapis alba L.）种子的子叶已被发现能够合成含有常见植物脂肪酸以及非常长链单不饱和（二十碳烯酸、芥酸和二十四碳烯酸）和饱和（二十烷酸、二十二烷酸和二十四烷酸）脂肪酸的脂质，这些脂肪酸来自各种放射性前体。来自放射性乙酸盐或丙二酸盐内源性合成的脂肪酸，或来自外源性添加的放射性脂肪酸的酰基脂质的体内标记模式表明，以下途径参与了三酰基甘油的生物合成。棕榈酸、硬脂酸和油酸在酰基辅酶 A（CoA）-途径中合成，并通过甘油-3-磷酸途径转化为三酰基甘油。硬脂酰-CoA 和油酰-CoA 池分别被延长为非常长链饱和和单不饱和酰基辅酶 A。大多数非常长链饱和酰基辅酶 A 酰化预先形成的二酰基甘油。非常长链单不饱和酰基辅酶 A 转化为三酰基甘油，部分通过磷脂酸和二酰基甘油，部分通过预先形成的二酰基甘油的酰化。
