Department of Biological Sciences, University of Idaho, Moscow, Idaho 83843.
Plant Physiol. 1984 Aug;75(4):1165-6. doi: 10.1104/pp.75.4.1165.
Evidence was obtained to support the hypothesis that ethylene is involved in xylem differentiation in primary pith explants of Lactuca sativa L. cv Romaine cultured in vitro. Xylem elements differentiated when explants were supplied indole-3-acetic acid (IAA) in combination with either the ethylene biosynthetic precursor 1-aminocyclopropane-1-carboxylic acid (ACC), the ethylene-releasing agent 2-chloroethylphosphonic acid (CEPA), or kinetin. In contrast, no xylem elements differentiated in the presence of IAA, kinetin, ACC, or CEPA alone, or when kinetin was supplied together with ACC or CEPA. These results show that ethylene will substitute qualitatively for cytokinin during auxin-induced xylogenesis, and suggest that both ethylene and auxin are required for xylem differentiation in Lactuca.
有证据表明，乙烯参与了生菜初级髓组织外植体的木质部分化，这些外植体在体外培养时用吲哚-3-乙酸（IAA）与乙烯生物合成前体 1-氨基环丙烷-1-羧酸（ACC）、乙烯释放剂 2-氯乙基膦酸（CEPA）或激动素一起供应。相比之下，当 IAA、激动素、ACC 或 CEPA 单独存在时，或当激动素与 ACC 或 CEPA 一起供应时，没有木质部元素分化。这些结果表明，乙烯在生长素诱导的木质部形成过程中可以替代细胞分裂素，并且表明乙烯和生长素都需要在生菜中进行木质部分化。
