Oberley L W, Bize I B, Sahu S K, Leuthauser S W, Gruber H E
J Natl Cancer Inst. 1978 Aug;61(2):375-9.
By means of both direct assay and gel electrophoresis, normal A/J mouse liver was shown to possess both Cu-Zn and Mn superoxide dismutase (SD) activity. H6 hepatoma cells contained Cu-Zn SD activity, but no Mn SD activity was detectable. Isolated mitochondria from normal liver contained both forms of the enzyme, but isolated mitochondria from H6 hepatoma cells contained no SD activity. To ascertain whether this loss of Mn SD activity was characteristic of these tumor cells or was simply a property of rapidly dividing cells, SD activity was measured in regenerating liver. Mn SD activity was present in the regenerating liver at all times after surgery. Hence loss of the Mn SD activity seemed to be a characteristic of some tumor cells but not of corresponding rapidly dividing normal cells.
通过直接测定法和凝胶电泳法发现，正常A/J小鼠肝脏同时具有铜锌超氧化物歧化酶（SD）和锰超氧化物歧化酶活性。H6肝癌细胞含有铜锌超氧化物歧化酶活性，但未检测到锰超氧化物歧化酶活性。从正常肝脏分离出的线粒体含有这两种形式的酶，但从H6肝癌细胞分离出的线粒体未检测到超氧化物歧化酶活性。为了确定锰超氧化物歧化酶活性的丧失是这些肿瘤细胞的特征还是仅仅是快速分裂细胞的特性，对再生肝脏中的超氧化物歧化酶活性进行了测量。手术后，再生肝脏中始终存在锰超氧化物歧化酶活性。因此，锰超氧化物歧化酶活性的丧失似乎是某些肿瘤细胞的特征，而不是相应快速分裂的正常细胞的特征。
