Department of Biological Sciences, University of Illinois at Chicago, Box 4348, Chicago, Illinois 60680.
Plant Physiol. 1985 Apr;77(4):807-10. doi: 10.1104/pp.77.4.807.
After dark-light transitions, there is a delay in photosynthetic CO(2) fixation by isolated pea chloroplasts in the range of some minutes. In order to assess the physiological significance of light modulation of enzyme activity in the control of induction, we made estimates of the kinetic parameters of fructose-1,6-bisphosphatase immediately upon release from pea chloroplasts in the dark and after illumination for various time periods. The Michaelis constant for fructose-1,6-bisphosphate decreased and maximal velocities increased during induction. It seems likely that light activation of this enzyme is one of the factors contributing to the overcoming of the lag period in photosynthetic CO(2) fixation.
在暗-光转变之后,分离的豌豆叶绿体在几分钟的范围内会延迟光合 CO₂固定。为了评估酶活性的光调节在诱导控制中的生理意义,我们在暗中从豌豆叶绿体中释放后以及经过不同时间的光照后,立即对果糖-1,6-二磷酸酶的动力学参数进行了估算。果糖-1,6-二磷酸的米氏常数在诱导过程中降低,最大速度增加。似乎光激活这种酶是克服光合 CO₂固定滞后期的因素之一。