在冬春小麦的低温驯化过程中，DNA 依赖性 RNA 聚合酶和核糖核酸酶对 RNA 合成的调控。
Regulation of RNA Synthesis by DNA-Dependent RNA Polymerases and RNases during Cold Acclimation in Winter and Spring Wheat.
机构信息
Departement des Sciences Biologiques, Universite du Quebec a Montreal, C.P. 8888, Succ. A, Montreal, Quebec, Canada H3C 3P8.
出版信息
Plant Physiol. 1985 Jun;78(2):250-5. doi: 10.1104/pp.78.2.250.
Chromatin DNA-dependent RNA polymerases and RNases activities were measured in winter and spring varieties to understand the overall regulation of RNA synthesis during cold acclimation. We found that total RNA polymerase activities were significantly higher in chromatin isolated from winter wheat compared to the spring wheat during the acclimation period. This increase was parallel to the increase in protein and RNA contents during hardening. The ratio of RNA polymerase I to RNA polymerase II activity was higher than 2 in winter wheat after 30 days of hardening compared, to a ratio of 0.90 under the nonhardening conditions. The increase in activity and the ratio of polymerase I to polymerase II was maintained after the separation of the enzymes from the template, suggesting that RNA synthesis is regulated in part at the enzyme level. On the other hand, the chromatin associated RNase activity decreased in both varieties during acclimation, indicating a nonspecific inhibition caused by low temperature rather than a selective genetic response associated with cold acclimation.
我们测定了冬季和春季品种的染色质 DNA 依赖性 RNA 聚合酶和 RNA 酶活性，以了解低温驯化过程中 RNA 合成的整体调控。我们发现，在驯化期间，从冬小麦中分离出的染色质中的总 RNA 聚合酶活性明显高于春小麦。这种增加与蛋白质和 RNA 含量的增加平行。在经过 30 天的硬化后，冬小麦中 RNA 聚合酶 I 与 RNA 聚合酶 II 的活性比高于 2，而在非硬化条件下，这一比值为 0.90。在从模板中分离出酶后，活性和聚合酶 I 与聚合酶 II 的比值增加，这表明 RNA 合成在一定程度上受到酶水平的调控。另一方面，在驯化过程中，两种品种的染色质相关的 RNA 酶活性都降低了，这表明低温引起的是一种非特异性抑制，而不是与低温驯化相关的选择性遗传反应。
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