Burke J J, Hatfield J L, Klein R R, Mullet J E
USDA Plant Stress and Water Conservation Research Unit, P. O. Box 4170, Texas Tech University, Lubbock, Texas 79409.
Plant Physiol. 1985 Jun;78(2):394-8. doi: 10.1104/pp.78.2.394.
Cotton (Gossypium hirsutum L.) plants grown under field water deficits exhibited an 80 to 85% reduction in leaf area index, plant height, and dry matter accumulation compared with irrigated controls. Midday photosynthetic rates of dryland plants decreased 2-fold, and canopy temperatures increased to 40 degrees C at 80 days after planting compared with canopy temperatures of 30 degrees C for irrigated plants. Leaves from dryland plants which had exhibited canopy temperatures of 40 degrees C for several weeks accumulated stainable levels of polypeptides with apparent molecular weights of 100, 94, 89, 75, 60, 58, 37, and 21 kilodaltons. These polypeptides did not accumulate in leaves from irrigated plants.Addition of [(35)S]methionine to leaves of growth chamber-grown cotton plants and subsequent incubation at 40 degrees C for 3 hours radiolabeled polypeptides with molecular weights similar to those that accumulate in dryland cotton leaves. These data suggest that the proteins which accumulate in water-stressed cotton leaves at elevated temperatures (40 degrees C) are heat shock proteins and that these proteins can accumulate to substantial levels in field-stressed plants.
与灌溉对照相比，在田间水分亏缺条件下种植的棉花（陆地棉）植株的叶面积指数、株高和干物质积累降低了80%至85%。与灌溉植株30℃的冠层温度相比，旱地植株的午间光合速率降低了2倍，种植80天后冠层温度升至40℃。冠层温度在40℃持续数周的旱地植株叶片积累了可染色水平的多肽，其表观分子量分别为100、94、89、75、60、58、37和21千道尔顿。这些多肽在灌溉植株的叶片中未积累。向生长室种植的棉花植株叶片中添加[³⁵S]甲硫氨酸，随后在40℃孵育3小时，对分子量与旱地棉花叶片中积累的多肽相似的多肽进行放射性标记。这些数据表明，在高温（40℃）下水分胁迫的棉花叶片中积累的蛋白质是热休克蛋白，并且这些蛋白质在田间胁迫的植株中可以积累到相当高的水平。
