CSIRO, Division of Plant Industry, G.P.O. Box 1600, Canberra, A.C.T. 1601, Australia.
Plant Physiol. 1985 Jul;78(3):561-7. doi: 10.1104/pp.78.3.561.
The effect of light quality on the composition, function and structure of the thylakoid membranes, as well as on the photosynthetic rates of intact fronds from Asplenium australasicum, a shade plant, grown in blue, white, or red light of equal intensity (50 microeinsteins per square meter per second) was investigated. When compared with those isolated from plants grown in white and blue light, thylakoids from plants grown in red light have higher chlorophyll a/chlorophyll b ratios and lower amounts of light-harvesting chlorophyll a/b-protein complexes than those grown in blue light. On a chlorophyll basis, there were higher levels of PSII reaction centers, cytochrome f and coupling factor activity in thylakoids from red light-grown ferns, but lower levels of PSI reaction centers and plastoquinone. The red light-grown ferns had a higher PSII/PSI reaction center ratio of 4.1 compared to 2.1 in blue light-grown ferns, and a larger apparent PSI unit size and a lower PSII unit size. The CO(2) assimilation rates in fronds from red light-grown ferns were lower on a unit area or fresh weight basis, but higher on a chlorophyll basis, reflecting the higher levels of electron carriers and electron transport in the thylakoids.The structure of thylakoids isolated from plants grown under the three light treatments was similar, with no significant differences in the number of thylakoids per granal stack or the ratio of appressed membrane length/nonappressed membrane length. The large freeze-fracture particles had the same size in the red-, blue-, and white-grown ferns, but there were some differences in their density. Light quality is an important factor in the regulation of the composition and function of thylakoid membranes, but the effects depend upon the plant species.
研究了光质对凤尾蕨（Asplenium australasicum）叶绿体膜的组成、功能和结构以及光合速率的影响，凤尾蕨是一种耐阴植物，在等强度的蓝光、白光和红光（50 微爱因斯坦/平方米/秒）下生长。与在白光和蓝光下生长的植株相比，在红光下生长的植株的叶绿体中叶绿素 a/叶绿素 b 的比值较高，收光叶绿素 a/b 蛋白复合物的含量较低。在叶绿素的基础上，在红光下生长的蕨类植物的类囊体中 PSII 反应中心、细胞色素 f 和偶联因子的活性较高，但 PSI 反应中心和质体醌的含量较低。与在蓝光下生长的蕨类植物相比，红光下生长的蕨类植物 PSII/PSI 反应中心的比值更高（4.1 对 2.1），PSI 单位尺寸更大，PSII 单位尺寸更小。红光下生长的蕨类植物的 CO2 同化速率在单位面积或鲜重基础上较低，但在叶绿素基础上较高，这反映了类囊体中电子载体和电子传递的水平较高。在三种光照处理下生长的植物中分离出的类囊体的结构相似，每个粒层堆栈的类囊体数量或压扁膜长度/非压扁膜长度的比例没有显著差异。在红光、蓝光和白光下生长的蕨类植物中，大的冷冻断裂颗粒的大小相同，但它们的密度存在一些差异。光质是调节类囊体膜组成和功能的重要因素，但这种影响取决于植物的种类。
