Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695.
Plant Physiol. 1985 Nov;79(3):914-9. doi: 10.1104/pp.79.3.914.
The ATPase subunit 6, located in the inner mitochondrial membrane, is encoded by mitochondrial genomes in animals and fungi. We have isolated and characterized a mitochondrial gene, designated atp 6, that encodes the subunit 6 polypeptide of Zea mays. Nucleotide and predicted amino acid sequence comparisons have revealed a homology of 44.6 and 33.2% with the yeast ATPase subunit 6 gene and polypeptide, respectively. The predicted protein in maize contains 291 amino acids with a molecular weight of 31,721. Hydropathy profiles generated for the maize and yeast polypeptides are very similar and contain large hydrophobic domains, characteristic of membrane bound proteins. RNA transfer blot analysis indicates that atp 6 is actively transcribed. Interestingly, 122 base pairs of nucleotide sequence interior to atp 6 have extensive homology with the 5' end of the cytochrome oxidase subunit II gene of maize mitochondria, suggesting recombination between the two genes.
位于线粒体内部膜的 ATP 酶亚基 6 由动物和真菌的线粒体基因组编码。我们已经分离并鉴定了一个线粒体基因，命名为 atp 6，它编码玉米的亚基 6 多肽。核苷酸和预测的氨基酸序列比较显示，与酵母 ATP 酶亚基 6 基因和多肽分别具有 44.6%和 33.2%的同源性。预测的玉米蛋白含有 291 个氨基酸，分子量为 31721。为玉米和酵母多肽生成的疏水性图谱非常相似，并且含有大的疏水区，这是膜结合蛋白的特征。RNA 转移印迹分析表明 atp 6 正在被转录。有趣的是，atp 6 内部的 122 个核苷酸序列与玉米线粒体细胞色素氧化酶亚基 II 基因的 5'端具有广泛的同源性，表明这两个基因之间发生了重组。
