Department of Agronomy, University of Florida, 32611, Gainesville, FL, USA.
Theor Appl Genet. 1988 Jul;76(1):25-32. doi: 10.1007/BF00288827.
Mitochondrial DNA (mtDNA) restriction endonuclease fragment patterns and patterns of mtDNA hybridized by mitochondrial gene probes were used to study phylogenetic relationships of seven Pennisetum species, including five P. americanum (pearl millet) ecotypes and a reference species from the distantly related genus, Panicum. The restriction patterns of the pearl millet ecotypes were uniform with the exception of the ecotype collected in Ethiopia. The probe hybridization method revealed more variability, with both the Rhodesian and Ethiopian ecotypes differing from the others and from each other. Considerable restriction pattern polymorphism was noted among different species of Pennisetum, and Panicum. Significant relationships were noted of Pennisetum polystachyon to P. pedicellatum and of P. purpureum to P. squamulatum using the restriction pattern method. In addition to those relationships, the hybridization method showed relationships of pearl millet to P. purpureum and to P. squamulatum. The relationships noted between species by the hybridization method agreed more closely to the cytological data than those indicated by the restriction pattern method. Therefore, the hybridization method appeared to be the preferred method for studying species relationships. The mitochondrial genome size of pearl millet was calculated to be 407 kb and the mitochondrial genome sizes of other Pennisetum species ranged from 341 to 486 kb.
线粒体 DNA（mtDNA）限制内切酶片段模式和线粒体基因探针杂交的模式被用于研究 7 种黍属物种的系统发育关系，包括 5 种珍珠粟（黍）生态型和一个来自远缘属的参考种。除了在埃塞俄比亚采集的生态型外，珍珠粟生态型的限制模式是统一的。探针杂交方法显示出更多的可变性，罗得西亚和埃塞俄比亚生态型与其他生态型和彼此之间都存在差异。在不同的黍属和黍属植物中都观察到了相当大的限制模式多态性。使用限制模式方法，注意到了 Pennisetum polystachyon 与 P. pedicellatum 和 P. purpureum 与 P. squamulatum 之间的显著关系。除了这些关系外，杂交方法还显示了珍珠粟与 P. purpureum 和 P. squamulatum 之间的关系。杂交方法所注意到的物种之间的关系比限制模式方法所指示的关系更接近细胞学数据。因此，杂交方法似乎是研究物种关系的首选方法。珍珠粟的线粒体基因组大小计算为 407kb，其他黍属植物的线粒体基因组大小范围为 341-486kb。
